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Product Details
23073-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
SUPT6H Fusion Protein Ag19426 (1618-1726 aa encoded by BC136524)
Conjugate
Form
Liquid
Concentration
0.29 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody specifically recognizes the 230 kDa phosphorylated SUPT6H protein.
Immunogen sequence: TPSYSYTTP SQPITTPQYH QLQASTTPQS AQAQPQPSSS SRQRQQQPKS NSHAAIDWGK MAEQWLQEKE AERRKQKQRL TPRPSPSPMI ESTPMSIAGD ATPLLDEMDR (1618-1726 aa encoded by BC136524)
Target Information
Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Histone chaperone suppressor of Ty6; hSPT6; MGC87943; suppressor of Ty 6 homolog; Tat-cotransactivator 2 protein; Tat-CT2 protein; Transcription elongation factor SPT6
Gene Aliases: 5131400N11Rik; AI132449; emb-5; KIAA0162; mKIAA0162; SPT6; SPT6H; Supt6; SUPT6H
UniProt ID: (Human) Q7KZ85, (Mouse) Q62383
Entrez Gene ID: (Human) 6830, (Mouse) 20926, (Rat) 303281
nucleobase-containing compound metabolic process
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
mRNA processing
transport
RNA splicing
regulation of mRNA export from nucleus
regulation of DNA-templated transcription, elongation
positive regulation of transcription elongation from RNA polymerase II promoter
regulation of isotype switching
regulation of mRNA processing
mRNA transport
regulation of muscle cell differentiation
negative regulation of histone H3-K27 methylation
chromatin remodeling
viral process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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