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SUV39H2 Antibody (PA5-120957) in WB
Western blot analysis of SUV39H2 in extracts of various cell lines. Samples were incubated with SUV39H2 Polyclonal antibody (Product # PA5-120957) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-120957
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 141-350 of human SUV39H2 (NP_001180354.1).
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914529
Product Specific Information
Positive test controls include: Mouse testis, Rat testis. The target is usually found in the following locations: Chromosome, Nucleus, centromere.
Immunogen sequence: CCPAEAGVLL AYNKNQQIKI PPGTPIYECN SRCQCGPDCP NRIVQKGTQY SLCIFRTSNG RGWGVKTLVK IKRMSFVMEY VGEVITSEEA ERRGQFYDNK GITYLFDLDY ESDEFTVDAA RYGNVSHFVN HSCDPNLQVF NVFIDNLDTR LPRIALFSTR TINAGEELTF DYQMKGSGDI SSDSIDHSPA KKRVRTVCKC GAVTCRGYLN
Target Information
The murine gene Suv39h2 encodes an H3 histone methyltransferase (HMTase) 59% identical in sequence to mouse Suv39h1. During embryogenesis, both proteins overlap in tissue expression, yet Suv39h2 transcripts are restricted to the testes in adult animals. Immunolocalization of the Suv39h2 protein during spermatogenesis indicates enrichment at the heterochromatin from the leptotene to the round spermatid stage. Moreover, Suv39h2 specifically accumulates with chromatin of the sex chromosomes, which undergo transcriptional silencing during the first meiotic prophase. Suv39h2 HMTase may also organize meiotic heterochromatin with the potential for epigenetic imprint to the male germline.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ23414; H3 K9 HMTase 2; H3-K9-HMTase 2; Histone H3-K9 methyltransferase 2; Histone-lysine N-methyltransferase SUV39H2; KMT1; Lysine N-methyltransferase 1B; RP11-2K17.2; Su; Su(var)3-9 homolog 2; Suppressor of variegation 3-9 homolog 2; Suv39h histone methyltransferase
Gene Aliases: 4930507K23Rik; AA536750; D030054H19Rik; D2Ertd544e; KMT1B; SUV39H2
UniProt ID: (Human) Q9H5I1, (Mouse) Q9EQQ0
Entrez Gene ID: (Human) 79723, (Rat) 364785, (Mouse) 64707
transcription regulatory region sequence-specific DNA binding
protein binding
zinc ion binding
histone-lysine N-methyltransferase activity
histone methyltransferase activity (H3-K9 specific)
RNA polymerase II regulatory region sequence-specific DNA binding
molecular_function
chromatin binding
methyltransferase activity
protein methyltransferase activity
transferase activity
metal ion binding
negative regulation of transcription from RNA polymerase II promoter
chromatin assembly or disassembly
chromatin remodeling
transcription, DNA-templated
cell cycle
cell differentiation
histone H3-K9 dimethylation
histone H3-K9 trimethylation
negative regulation of circadian rhythm
negative regulation of transcription, DNA-templated
rhythmic process
cellular response to hypoxia
male meiosis
biological_process
peptidyl-lysine methylation
histone lysine methylation
histone H3-K9 methylation
regulation of transcription, DNA-templated
chromatin modification
methylation
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