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SUZ12 Antibody (MA5-11188) in ICC/IF
Immunofluorescence analysis of SUZ12 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SUZ12 Monoclonal Antibody (K.6.9) (Product # MA5-11188) at 1:800 dilution in 0.1% BSA, incubated at 4-degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produc... View More
Product Details
MA5-11188
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
K.6.9
Immunogen
Synthetic peptide corresponding to the sequence of the human SUZ12 protein
Conjugate
Form
Liquid
Concentration
84 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10987451
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
SUZ12 (polycomb protein SUZ12) is a zinc finger gene and has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. SUZ12 is also a component of the PRC2/EED-EZH2 complex which methylates Lys-9 (H3K9me) and Lys-27 (H3K27me) of histone H3 leading to transcriptional repression of the affected target gene. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ChET 9 protein; Chromatin precipitated E2F target 9 protein; Joined to JAZF1 protein; Polycomb protein SUZ12; Suppressor of zeste 12 protein homolog; SUZ12 polycomb repressive complex 2 subunit
Gene Aliases: 2610028O16Rik; AI195385; AU016842; AW536442; CHET9; D11Ertd530e; JJAZ1; KIAA0160; mKIAA0160; SUZ12
UniProt ID: (Human) Q15022, (Mouse) Q80U70
Entrez Gene ID: (Human) 23512, (Mouse) 52615, (Rat) 688041
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of cell proliferation
chromatin modification
histone methylation
histone ubiquitination
negative regulation of chemokine production
negative regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of cell differentiation
negative regulation of epithelial cell proliferation
histone H3-K27 trimethylation
spinal cord development
response to retinoic acid
negative regulation of gene expression, epigenetic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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