Proteintech

SYVN1 Polyclonal Antibody

SYVN1 Antibody in Immunocytochemistry (ICC/IF)

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SYVN1 Antibody (13473-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 13473-1-AP (SYVN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SYVN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SYVN1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13473-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 35 publications 35 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 5 publications 5 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SYVN1 Fusion Protein Ag4267 (242-617 aa encoded by BC030530)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LFAIRPMYL AMRQFKKAVT DAIMSRRAIR NMNTLYPDAT PEELQAMDNV CIICREEMVT GAKRLPCNHI FHTSCLRSWF QRQQTCPTCR MDVLRASLPA QSPPPPEPAD QGPPPAPHPP PLLPQPPNFP QGLLPPFPPG MFPLWPPMGP FPPVPPPPSS GEAVAPPSTS AAALSRPSGA ATTTAAGTSA TAASATASGP GSGSAPEAGP APGFPFPPPW MGMPLPPPFA FPPMPVPPAG FAGLTPEELR ALEGHERQHL EARLQSLRNI HTLLDAAMLQ INQYLTVLAS LGPPRPATSV NSTEETATTV VAAASSTSIP SSEATTPTPG ASPPAPEMER PPAPESVGTE EMPEDGEPDA AELRRRRLQK LESPVAH (242-617 aa encoded by BC030530 )

Target Information

HRD1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of HRD1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. HRD1 is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. It has been reported that Synoviolin/Hrd1 (expressed in rheumatoid synovium) is a novel causative factor for arthropathy by triggering synovial cell outgrowth through its anti-apoptotic effects. HRD1 contains one ring-type zinc finger.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Characterization of dietary and herbal sourced natural compounds that modulate SEL1L-HRD1 ERAD activity and alleviate protein misfolding in the ER.

The Journal of nutritional biochemistry

Yang J,Zhi Y,Wen S,Pan X,Wang H,He X,Lu Y,Zhu Y,Chen Y,Shi G

Sun Jan 01 00:00:00 UTC 2023

The Brucella abortus Type IV Effector BspA Inhibits MARCH6-Dependent ERAD To Promote Intracellular Growth.

Infection and immunity

Kambarev S,Borghesan E,Miller CN,Myeni S,Celli J

Tue May 16 00:00:00 UTC 2023

SEL1L-HRD1 endoplasmic reticulum-associated degradation controls STING-mediated innate immunity by limiting the size of the activable STING pool.

Nature cell biology

Ji Y,Luo Y,Wu Y,Sun Y,Zhao L,Xue Z,Sun M,Wei X,He Z,Wu SA,Lin LL,Lu Y,Chang L,Chen F,Chen S,Qian W,Xu X,Chen S,Pan D,Zhou Z,Xia S,Hu CA,Liang T,Qi L

Mon May 01 00:00:00 UTC 2023

Pharmacological chaperones restore proteostasis of epilepsy-associated GABA_A receptor variants.

bioRxiv : the preprint server for biology

Wang YJ,Seibert H,Ahn LY,Schaffer AE,Mu TW

Wed Apr 19 00:00:00 UTC 2023

The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.

Nature communications

Ganji R,Paulo JA,Xi Y,Kline I,Zhu J,Clemen CS,Weihl CC,Purdy JG,Gygi SP,Raman M

Mon Feb 06 00:00:00 UTC 2023

Perturbation of endoplasmic reticulum proteostasis triggers tissue injury in the thyroid gland.

JCI insight

Zhang X,Young C,Liao XH,Refetoff S,Torres M,Tomer Y,Stefan-Lifshitz M,Zhang H,Larkin D,Fang D,Qi L,Arvan P

Thu Jun 22 00:00:00 UTC 2023

Perturbation of endoplasmic reticulum proteostasis triggers tissue injury in the thyroid gland.

JCI insight

Zhang X,Young C,Liao XH,Refetoff S,Torres M,Tomer Y,Stefan-Lifshitz M,Zhang H,Larkin D,Fang D,Qi L,Arvan P

Thu Jun 22 00:00:00 UTC 2023

Hepatic SEL1L-HRD1 ER-associated degradation regulates systemic iron homeostasis via ceruloplasmin.

Proceedings of the National Academy of Sciences of the United States of America

Thepsuwan P,Bhattacharya A,Song Z,Hippleheuser S,Feng S,Wei X,Das NK,Sierra M,Wei J,Fang D,Huang YM,Zhang K,Shah YM,Sun S

Tue Jan 10 00:00:00 UTC 2023

The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes.

Nature communications

Wu SA,Shen C,Wei X,Zhang X,Wang S,Chen X,Torres M,Lu Y,Lin LL,Wang HH,Hunter AH,Fang D,Sun S,Ivanova MI,Lin Y,Qi L

Tue May 30 00:00:00 UTC 2023

AAA-ATPase valosin-containing protein binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4.

The Journal of biological chemistry

Shibuya K,Ebihara K,Ebihara C,Sawayama N,Isoda M,Yamamuro D,Takahashi M,Nagashima S,Ishibashi S

Wed Jun 01 00:00:00 UTC 2022

Endoplasmic reticulum stress induces mitochondrial dysfunction but not mitochondrial unfolded protein response in SH-SY5Y cells.

Molecular and cellular biochemistry

Evinova A,Hatokova Z,Tatarkova Z,Brodnanova M,Dibdiakova K,Racay P

Tue Mar 01 00:00:00 UTC 2022

Bombesin receptor-activated protein exacerbates cisplatin-induced AKI by regulating the degradation of SIRT2.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Peng L,Liu D,Liu H,Xia M,Wan L,Li M,Zhao J,Tang C,Chen G,Qu X,Dong Z,Liu H

Wed Nov 23 00:00:00 UTC 2022

Bombesin receptor-activated protein exacerbates cisplatin-induced AKI by regulating the degradation of SIRT2.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Peng L,Liu D,Liu H,Xia M,Wan L,Li M,Zhao J,Tang C,Chen G,Qu X,Dong Z,Liu H

Wed Nov 23 00:00:00 UTC 2022

Sigma-1 receptor attenuates osteoclastogenesis by promoting ER-associated degradation of SERCA2.

EMBO molecular medicine

Wei X,Zheng Z,Feng Z,Zheng L,Tao S,Zheng B,Huang B,Zhang X,Liu J,Chen Y,Zong W,Shan Z,Fan S,Chen J,Zhao F

Thu Jul 07 00:00:00 UTC 2022

S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.

Cellular and molecular life sciences : CMLS

Sun Y,Fan Y,Wang Z,Li M,Su D,Liu Y,Liang X

Sat Mar 12 00:00:00 UTC 2022

S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.

Cellular and molecular life sciences : CMLS

Sun Y,Fan Y,Wang Z,Li M,Su D,Liu Y,Liang X

Sat Mar 12 00:00:00 UTC 2022

S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.

Cellular and molecular life sciences : CMLS

Sun Y,Fan Y,Wang Z,Li M,Su D,Liu Y,Liang X

Sat Mar 12 00:00:00 UTC 2022

Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function.

The Journal of clinical investigation

Yoshida S,Wei X,Zhang G,O'Connor CL,Torres M,Zhou Z,Lin L,Menon R,Xu X,Zheng W,Xiong Y,Otto E,Tang CA,Hua R,Verma R,Mori H,Zhang Y,Hu CA,Liu M,Garg P,Hodgin JB,Sun S,Bitzer M,Qi L

Thu Apr 01 00:00:00 UTC 2021

THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.

Journal for immunotherapy of cancer

Li C,Chi H,Deng S,Wang H,Yao H,Wang Y,Chen D,Guo X,Fang JY,He F,Xu J

Sun Aug 01 00:00:00 UTC 2021

THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.

Journal for immunotherapy of cancer

Li C,Chi H,Deng S,Wang H,Yao H,Wang Y,Chen D,Guo X,Fang JY,He F,Xu J

Sun Aug 01 00:00:00 UTC 2021

Hydrogen sulphide reduced the accumulation of lipid droplets in cardiac tissues of db/db mice via Hrd1 S-sulfhydration.

Journal of cellular and molecular medicine

Sun Y,Zhang L,Lu B,Wen J,Wang M,Zhang S,Li Q,Shu F,Lu F,Liu N,Peng S,Zhao Y,Dong S,Lu F,Zhang W,Wang Y

Fri Oct 01 00:00:00 UTC 2021

PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones.

Nature communications

Chen YJ,Knupp J,Arunagiri A,Haataja L,Arvan P,Tsai B

Wed Oct 13 00:00:00 UTC 2021

Preservation of endoplasmic reticulum (ER) Ca²⁺ stores by deletion of inositol-1,4,5-trisphosphate receptor type 1 promotes ER retrotranslocation, proteostasis, and protein outer segment localization in cyclic nucleotide-gated channel-deficient cone photoreceptors.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yang F,Ma H,Butler MR,Ding XQ

Tue Jun 01 00:00:00 UTC 2021

STING regulates BCR signaling in normal and malignant B cells.

Cellular & molecular immunology

Tang CA,Lee AC,Chang S,Xu Q,Shao A,Lo Y,Spalek WT,Pinilla-Ibarz JA,Del Valle JR,Hu CA

Thu Apr 01 00:00:00 UTC 2021

SYVN1-MTR4-MAT2A Signaling Axis Regulates Methionine Metabolism in Glioma Cells.

Frontiers in cell and developmental biology

Wang L,Hu B,Pan K,Chang J,Zhao X,Chen L,Lin H,Wang J,Zhou G,Xu W,Yuan J

Fri Apr 22 00:00:00 UTC 2022

SYVN1-MTR4-MAT2A Signaling Axis Regulates Methionine Metabolism in Glioma Cells.

Frontiers in cell and developmental biology

Wang L,Hu B,Pan K,Chang J,Zhao X,Chen L,Lin H,Wang J,Zhou G,Xu W,Yuan J

Fri Apr 22 00:00:00 UTC 2022

Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway.

Acta pharmacologica Sinica

Dong JY,Xia KJ,Liang W,Liu LL,Yang F,Fang XS,Xiong YJ,Wang L,Zhou ZJ,Li CY,Zhang WD,Wang JY,Chen DP

Wed Sep 01 00:00:00 UTC 2021

Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway.

Acta pharmacologica Sinica

Dong JY,Xia KJ,Liang W,Liu LL,Yang F,Fang XS,Xiong YJ,Wang L,Zhou ZJ,Li CY,Zhang WD,Wang JY,Chen DP

Wed Sep 01 00:00:00 UTC 2021

SEL1L degradation intermediates stimulate cytosolic aggregation of polyglutamine-expanded protein.

The FEBS journal

Hattori T,Hanafusa K,Wada I,Hosokawa N

Sun Aug 01 00:00:00 UTC 2021

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Molecular cancer

Fan Y,Wang J,Jin W,Sun Y,Xu Y,Wang Y,Liang X,Su D

Tue Feb 02 00:00:00 UTC 2021

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Molecular cancer

Fan Y,Wang J,Jin W,Sun Y,Xu Y,Wang Y,Liang X,Su D

Tue Feb 02 00:00:00 UTC 2021

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Molecular cancer

Fan Y,Wang J,Jin W,Sun Y,Xu Y,Wang Y,Liang X,Su D

Tue Feb 02 00:00:00 UTC 2021

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Developmental cell

Ran J,Liu M,Feng J,Li H,Ma H,Song T,Cao Y,Zhou P,Wu Y,Yang Y,Yang Y,Yu F,Guo H,Zhang L,Xie S,Li D,Gao J,Zhang X,Zhu X,Zhou J

Mon May 04 00:00:00 UTC 2020

Cross-talks of glycosylphosphatidylinositol biosynthesis with glycosphingolipid biosynthesis and ER-associated degradation.

Nature communications

Wang Y,Maeda Y,Liu YS,Takada Y,Ninomiya A,Hirata T,Fujita M,Murakami Y,Kinoshita T

Thu Feb 13 00:00:00 UTC 2020

Exogenous H₂S Induces Hrd1 S-sulfhydration and Prevents CD36 Translocation via VAMP3 Ubiquitylation in Diabetic Hearts.

Aging and disease

Yu M,Du H,Wang B,Chen J,Lu F,Peng S,Sun Y,Liu N,Sun X,Shiyun D,Zhao Y,Wang Y,Zhao D,Lu F,Zhang W

Wed Apr 01 00:00:00 UTC 2020

Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.

Molecular cell

Cunningham CN,Williams JM,Knupp J,Arunagiri A,Arvan P,Tsai B

Thu Aug 08 00:00:00 UTC 2019

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Cell metabolism

Luo H,Jiang M,Lian G,Liu Q,Shi M,Li TY,Song L,Ye J,He Y,Yao L,Zhang C,Lin ZZ,Zhang CS,Zhao TJ,Jia WP,Li P,Lin SY,Lin SC

Tue Apr 03 00:00:00 UTC 2018

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Cell metabolism

Luo H,Jiang M,Lian G,Liu Q,Shi M,Li TY,Song L,Ye J,He Y,Yao L,Zhang C,Lin ZZ,Zhang CS,Zhao TJ,Jia WP,Li P,Lin SY,Lin SC

Tue Apr 03 00:00:00 UTC 2018

Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation.

Molecular cell

van der Goot AT,Pearce MMP,Leto DE,Shaler TA,Kopito RR

Thu May 03 00:00:00 UTC 2018

Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation.

Molecular cell

van der Goot AT,Pearce MMP,Leto DE,Shaler TA,Kopito RR

Thu May 03 00:00:00 UTC 2018

The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.

The Journal of biological chemistry

Saldate JJ,Shiau J,Cazares VA,Stuenkel EL

Fri Feb 16 00:00:00 UTC 2018

Endoplasmic Reticulum Stress Induces HRD1 to Protect Alveolar Type II Epithelial Cells from Apoptosis Induced by Cigarette Smoke Extract.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Tan SX,Jiang DX,Hu RC,Dai AG,Gan GX,Fu DY,Kong CC,Chen YR,Wang LL,Li J

Mon Dec 18 00:00:00 UTC 2017

Endoplasmic Reticulum Stress Induces HRD1 to Protect Alveolar Type II Epithelial Cells from Apoptosis Induced by Cigarette Smoke Extract.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Tan SX,Jiang DX,Hu RC,Dai AG,Gan GX,Fu DY,Kong CC,Chen YR,Wang LL,Li J

Mon Dec 18 00:00:00 UTC 2017

Characterization of protein complexes of the endoplasmic reticulum-associated degradation E3 ubiquitin ligase Hrd1.

The Journal of biological chemistry

Hwang J,Walczak CP,Shaler TA,Olzmann JA,Zhang L,Elias JE,Kopito RR

Fri Jun 02 00:00:00 UTC 2017

Characterization of protein complexes of the endoplasmic reticulum-associated degradation E3 ubiquitin ligase Hrd1.

The Journal of biological chemistry

Hwang J,Walczak CP,Shaler TA,Olzmann JA,Zhang L,Elias JE,Kopito RR

Fri Jun 02 00:00:00 UTC 2017

PDI reductase acts on Akita mutant proinsulin to initiate retrotranslocation along the Hrd1/Sel1L-p97 axis.

Molecular biology of the cell

He K,Cunningham CN,Manickam N,Liu M,Arvan P,Tsai B

Thu Oct 01 00:00:00 UTC 2015

A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.

PLoS pathogens

Walczak CP,Ravindran MS,Inoue T,Tsai B

Sat Mar 01 00:00:00 UTC 2014

A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.

PLoS pathogens

Walczak CP,Ravindran MS,Inoue T,Tsai B

Sat Mar 01 00:00:00 UTC 2014

The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation.

Molecular biology of the cell

Williams JM,Inoue T,Banks L,Tsai B

Fri Mar 01 00:00:00 UTC 2013

The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation.

Molecular biology of the cell

Williams JM,Inoue T,Banks L,Tsai B

Fri Mar 01 00:00:00 UTC 2013

A deubiquitinase negatively regulates retro-translocation of nonubiquitinated substrates.

Molecular biology of the cell

Bernardi KM,Williams JM,Inoue T,Schultz A,Tsai B

Fri Nov 01 00:00:00 UTC 2013

A deubiquitinase negatively regulates retro-translocation of nonubiquitinated substrates.

Molecular biology of the cell

Bernardi KM,Williams JM,Inoue T,Schultz A,Tsai B

Fri Nov 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog; HRD1 protein; MGC40372; RING-type E3 ubiquitin transferase synoviolin; Synovial apoptosis inhibitor 1; synovial apoptosis inhibitor 1, synoviolin; synoviolin 1

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Gene Aliases: 1200010C09Rik; AW211966; C85322; D530017H19Rik; DER3; HRD1; KIAA1810; RGD1310488; SYVN1

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UniProt ID: (Human) Q86TM6, (Mouse) Q9DBY1

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Entrez Gene ID: (Human) 84447, (Mouse) 74126, (Rat) 361712

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Function(s)

protein binding zinc ion binding ligase activity unfolded protein binding chaperone binding ATPase binding ubiquitin protein ligase activity ubiquitin protein ligase activity involved in ERAD pathway ubiquitin-specific protease binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity activating transcription factor binding coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity acid-amino acid ligase activity

Process(es)

in utero embryonic development response to unfolded protein multicellular organism development protein ubiquitination protein N-linked glycosylation via asparagine protein catabolic process ER-associated ubiquitin-dependent protein catabolic process retrograde protein transport, ER to cytosol negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway endoplasmic reticulum unfolded protein response IRE1-mediated unfolded protein response ERAD pathway protein ubiquitination involved in ubiquitin-dependent protein catabolic process protein stabilization protein K48-linked ubiquitination endoplasmic reticulum mannose trimming

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SYVN1 Polyclonal Antibody

SYVN1 Antibody (13473-1-AP) in ICC/IF

Product Details

13473-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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