Invitrogen

Sall1 Monoclonal Antibody (NRNSTNX), eFluor™ 450, eBioscience™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

48-9729-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

1.0 µg/test
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NRNSTNX

Immunogen

C-terminal fragment of the protein (aa-805-1322)
View immunogen

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848393

Product Specific Information

Description: This NRNSTNX monoclonal antibody recognizes mouse Sall1.

Applications Reported: This NRNSTNX antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This NRNSTNX antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse brain cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols . This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Target Information

Sall1, which encodes a zinc finger protein, functions as a transcriptional repressor and interacts physically with histone deacetylase and other components of the chromatin remodeling NuRD complex. It is unknown whether the transcriptional repression is solely dependent on histone deacetylase activity. Gene expression profiling has identified Sall1 as a microglial signature gene. Microglia are the resident macrophages of the central nervous system (CNS). Sall1 is also expressed in abundance in the mesenchyme-derived structure from condensed mesenchyme, S-comma-shaped bodies, to renal tubules and podocytes. Sall1 has been identified as a key transcription factor in self-renewal renal progenitor cells. Sall1 is required to maintain the stemness of nephron progenitor cells by restraining their differentiation into renal vesicles. Defects in SALL1 are the cause of Townes-Brocks syndrome as well as bronchio-oto-renal syndrome. Heterozygous mutations of human SALL1 leading to Townes-Brocks syndrome features dysplastic ears, preaxial polydactyly, imperforate anus, and less commonly, kidney and heart anomalies (Kohlhase et al. 1998). Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: sal-3; sal-like protein 1; zinc finger protein Spalt-3

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Gene Aliases: Msal-3

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Entrez Gene ID: (Mouse) 58198

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding RNA polymerase II transcription coactivator activity enhancer sequence-specific DNA binding nucleic acid binding DNA binding chromatin binding histone deacetylase activity protein binding beta-catenin binding metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ureteric bud development branching involved in ureteric bud morphogenesis kidney development neural tube closure ventricular septum development mesenchymal to epithelial transition involved in metanephros morphogenesis negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter heart development olfactory nerve development olfactory bulb development olfactory bulb interneuron differentiation neural tube development positive regulation of Wnt signaling pathway inductive cell-cell signaling forelimb morphogenesis hindlimb morphogenesis outer ear morphogenesis embryonic digit morphogenesis positive regulation of neuron differentiation negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic digestive tract development limb development olfactory bulb mitral cell layer development kidney epithelium development ureteric bud invasion mesenchymal stem cell maintenance involved in metanephric nephron morphogenesis positive regulation of branching involved in ureteric bud morphogenesis regulation of neural precursor cell proliferation negative regulation of mesoderm development negative regulation of ectoderm development
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