Invitrogen

Sarcalumenin Monoclonal Antibody (XIIC4)

Sarcalumenin Antibody in Western Blot (WB)

FIGURE: 1 / 5

Sarcalumenin Antibody (MA3-932) in WB

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Skeletal Muscle (Lane 3), Rat Skeletal Muscle (Lane 4), Mouse Kidney (Lane 5), Rat Kidney (Lane 6), Rat Lung (Lane 7), Rat Pancreas (Lane 8), Rat Stomach (Lane 9) and Rat Adipose (Lane 10). The blot was probed with Sarcalumenin Mouse Monoclonal Antibody (Product # MA3-932, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/m... View More

Product Image Gallery

Product Details

MA3-932

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1/500

Product Specifications

Species Reactivity

Dog, Guinea pig, Mouse, Rabbit, Rat

Published species

Dog, Human, Mouse, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

XIIC4

Immunogen

Purified rabbit sarcoplasmic reticulum membrane vesicles.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2194562

Product Specific Information

MA3-932 detects sarcalumenin from canine, guinea pig, mouse and rabbit tissues. This antibody specifically detects sarcalumenin and the 53 kDa glycoprotein splice variant.

MA3-932 has been successfully used in Western blot. By Western blot, this antibody detects a 53 kDa and a 160 kDa protein representing the glycoprotein and sarcalumenin, respectively, in rabbit skeletal muscle extracts.

The MA3-932 antigen is purified rabbit sarcoplasmic reticulum membrane vesicles.

Target Information

Sarcalumenin is a 160 kDa acidic calcium binding glycoprotein found to reside at the inner membrane of the sarcoplasmic reticulum in a calcium dependent manner. The gene encoding sarcalumenin also encodes an alternate splice variant, a 53 kDa glycoprotein which co-localizes with sarcalumenin. These two proteins are found in the longitudinal sarcoplasmic reticulum and the nonjunctional membranes of the terminal cisternae. They have been observed to co-localize with calcium-ATPase suggesting that they are involved in calcium transport and sequestration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative reduction of RyR1 protein caused by a single-allele frameshift mutation in RYR1 ex36 impairs the strength of adult skeletal muscle fibres.

Human molecular genetics

Elbaz M,Ruiz A,Eckhardt J,Pelczar P,Muntoni F,Boncompagni S,Treves S,Zorzato F

MA3-932 was used in Western Blotting to characterise a mouse model knocked-in for a frameshift mutation in RYR1 exon 36 (p.Gln1970fsX16) that is isogenic to that identified in one parent of a severely affected patient with recessively inherited multiminicore disease.

Sat Jun 01 00:00:00 UTC 2019

Phospholamban is concentrated in the nuclear envelope of cardiomyocytes and involved in perinuclear/nuclear calcium handling.

Journal of molecular and cellular cardiology

Wu AZ,Xu D,Yang N,Lin SF,Chen PS,Cala SE,Chen Z

MA3932 was used in immunohistochemistry and western blot to investigate the localization of phopholamban in cardiomyocites.

Tue Nov 01 00:00:00 UTC 2016

Functional characterization of orbicularis oculi and extraocular muscles.

The Journal of general physiology

Sekulic-Jablanovic M,Ullrich ND,Goldblum D,Palmowski-Wolfe A,Zorzato F,Treves S

MA3-932 was used in western blot to assess the function and characterization of extraocular muscles and obicularis oculi

Sun May 01 00:00:00 UTC 2016

Role of the JP45-Calsequestrin Complex on Calcium Entry in Slow Twitch Skeletal Muscles.

The Journal of biological chemistry

Mosca B,Eckhardt J,Bergamelli L,Treves S,Bongianino R,De Negri M,Priori SG,Protasi F,Zorzato F

MA3-932 was used in western blot to analyze slow twitch skeletal muscles and the role of the JP45-calsequestrin complex on calcium entry

Fri Jul 08 00:00:00 UTC 2016

Characterization of excitation-contraction coupling components in human extraocular muscles.

The Biochemical journal

Sekulic-Jablanovic M,Palmowski-Wolfe A,Zorzato F,Treves S

MA3-932 was used in western blot to study the role of RyR in regulating calcium levels in extraocular muscles.

Sun Feb 15 00:00:00 UTC 2015

Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy.

Neuromuscular disorders : NMD

Di Blasi C,Blasevich F,Bellafiore E,Mottarelli E,Gibertini S,Zanotti S,Saredi S,Mantegazza R,Morandi L,Mora M

MA3-932 was used in immunohistochemistry to investigate the immunohistochemical features of hexagonally cross-linked tubular arrays myopathy

Sat May 01 00:00:00 UTC 2010

Reduced expression of regucalcin in young and aged mdx diaphragm indicates abnormal cytosolic calcium handling in dystrophin-deficient muscle.

Biochimica et biophysica acta

Doran P,Dowling P,Donoghue P,Buffini M,Ohlendieck K

MA3-932 was used in western blot to study the reduced regucalcin expression observed in diaphragm muscle in muscular dystrophy and the significance for calcium handling

Sat Apr 01 00:00:00 UTC 2006

The origin of tubular aggregates in human myopathies.

The Journal of pathology

Chevessier F,Bauché-Godard S,Leroy JP,Koenig J,Paturneau-Jouas M,Eymard B,Hantaï D,Verdière-Sahuqué M

MA3-932 was used in immunohistochemistry to study the origin and significance of tubular aggregates in human myopathies

Tue Nov 01 00:00:00 UTC 2005

Glycogen- and PP1c-targeting subunit GM is phosphorylated at Ser48 by sarcoplasmic reticulum-bound Ca2+-calmodulin protein kinase in rabbit fast twitch skeletal muscle.

The Journal of biological chemistry

Sacchetto R,Bovo E,Donella-Deana A,Damiani E

MA3-932 was used in western blot to investigate the role of the SR-bound CaMKII during the regulation of GS activity

Fri Feb 25 00:00:00 UTC 2005

Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle.

Journal of applied physiology (Bethesda, Md. : 1985)

Glover L,Heffron JJ,Ohlendieck K

MA3-932 was used in western blot to investigate the importance of RyR2 complex formation in the development of a metabolic crisis in malignant hyperthermia

Thu Jan 01 00:00:00 UTC 2004

Subproteomics analysis of Ca+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle.

European journal of biochemistry

Doran P,Dowling P,Lohan J,McDonnell K,Poetsch S,Ohlendieck K

MA3-932 was used in western blot to study the reduced expression of calsequestrin in dystrophic mouse skeletal muscle

Fri Oct 01 00:00:00 UTC 2004

Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers.

Biochimica et biophysica acta

Froemming GR,Murray BE,Harmon S,Pette D,Ohlendieck K

MA3-932 was used in western blot to study the expression of Ca(2+) regulatory membrane protein isoforms in skeletal, cardiac and neonatal muscle fibres

Thu Jun 01 00:00:00 UTC 2000

Effects of chronic low-frequency stimulation on Ca2+-regulatory membrane proteins in rabbit fast muscle.

Pflugers Archiv : European journal of physiology

Ohlendieck K,Frömming GR,Murray BE,Maguire PB,Leisner E,Traub I,Pette D

MA3-932 was used in western blot to study the effects of chronic low frequency stimulation of fast-twitch muscle on the levels of Ca(2+) regulatory membrane proteins

Fri Oct 01 00:00:00 UTC 1999

Analysis of excitation-contraction-coupling components in chronically stimulated canine skeletal muscle.

European journal of biochemistry

Ohlendieck K,Briggs FN,Lee KF,Wechsler AW,Campbell KP

MA3-932 was used in western blot to investigate the protein components of skeletal muscle membranes in canine muscle

Wed Dec 18 00:00:00 UTC 1991

Purification, calcium binding properties, and ultrastructural localization of the 53,000- and 160,000 (sarcalumenin)-dalton glycoproteins of the sarcoplasmic reticulum.

The Journal of biological chemistry

Leberer E,Timms BG,Campbell KP,MacLennan DH

MA3-932 was used in western blot to define properties and structural peculiarities of two sarcoplasmic reticulum glycoproteins

Fri Jun 15 00:00:00 UTC 1990

Bioinformatics

Protein Aliases: Sarcalumenin; sarcolumenin; sarcoplasmic reticulum glycoprotein

View more View less

Gene Aliases: 9830004M20Rik; AW108387; Sar; SRL

View more View less

UniProt ID: (Rabbit) P13666, (Mouse) Q7TQ48

View more View less

Entrez Gene ID: (Dog) 490030, (Rabbit) 100009112, (Mouse) 106393, (Rat) 302948

View more View less

Function(s)

GTP binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Sarcalumenin Monoclonal Antibody (XIIC4)

Sarcalumenin Antibody (MA3-932) in WB

Product Details

MA3-932

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!