Invitrogen

Sarcomeric alpha Actinin Monoclonal Antibody (EA-53)

Sarcomeric alpha Actinin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Sarcomeric alpha Actinin Antibody (MA1-22863) in IHC (P)

Immunohistochemical (Paraffin) analysis of human skeletal muscle tissue using (Product # MA1-22863) Sarcomeric alpha Actinin Monoclonal Antibody (EA-53).

Sarcomeric alpha Actinin Antibody in Western Blot (WB)

Sarcomeric alpha Actinin Antibody in Immunocytochemistry (ICC/IF)

Sarcomeric alpha Actinin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

MA1-22863

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:30

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunohistochemistry - Free Floating (IHC (Free))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

View 7 publications 7 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Zebrafish

Published species

Human, Mouse, Pig, Rat, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

EA-53

Immunogen

Purified rabbit skeletal alpha-actinin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

Ascites

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_557426

Product Specific Information

MA1-22863 detects sarcomeric alpha Actinin in bovine, feline, chicken, fish, goat, hamster, lizard, mouse porcine, rabbit, rat, ovine, snake, and Xenopus laevis samples.

Target Information

This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A simple and scalable 3D printing methodology for generating aligned and extended human and murine skeletal muscle tissues.

Biomedical materials (Bristol, England)

Cakal SD,Radeke C,Alcala JF,Ellman DG,Butdayev S,Andersen DC,Calloe K,Lind JU

MA1-22863 was used in Immunocytochemistry, Immunohistochemistry - Free Floating, Immunohistochemistry (Paraffin) to find high-resolution patterning is not required, as even patterns with feature sizes of several hundred micrometers is sufficient.

Tue May 17 00:00:00 UTC 2022

Recapitulating human cardio-pulmonary co-development using simultaneous multilineage differentiation of pluripotent stem cells.

eLife

Ng WH,Johnston EK,Tan JJ,Bliley JM,Feinberg AW,Stolz DB,Sun M,Wijesekara P,Hawkins F,Kotton DN,Ren X

MA1-22863 was used in Immunocytochemistry to report a novel stepwise strategy for directing the simultaneous induction of both mesoderm-derived cardiac and endoderm-derived lung epithelial lineages within a single differentiation of human-induced pluripotent stem cells (hiPSCs) via temporal specific tuning of WNT and nodal signaling in the absence of exogenous growth factors.

Wed Jan 12 00:00:00 UTC 2022

Cardiac ultrastructure inspired matrix induces advanced metabolic and functional maturation of differentiated human cardiomyocytes.

Cell reports

Afzal J,Liu Y,Du W,Suhail Y,Zong P,Feng J,Ajeti V,Sayyad WA,Nikolaus J,Yankova M,Deymier AC,Yue L

MA1-22863 was used in Flow cytometry/Cell sorting to present a method to accelerate hiPSC-CM maturation on a substrate, cardiac mimetic matrix (CMM), mimicking adult human heart matrix ligand chemistry, rigidity, and submicron ultrastructure, which synergistically mature hiPSC-CMs rapidly within 30 days.

Tue Jul 26 00:00:00 UTC 2022

Pre-clinical dose-escalation studies establish a therapeutic range for U7snRNA-mediated DMD exon 2 skipping.

Molecular therapy. Methods & clinical development

Simmons TR,Vetter TA,Huang N,Vulin-Chaffiol A,Wein N,Flanigan KM

MA1-22863 was used in Western Blot to develop an adeno-associated virus (AAV)-based U7snRNA exon-skipping approach directed toward exon 2, duplications of which represent 10% of all DMD duplication mutations.

Fri Jun 11 00:00:00 UTC 2021

Large-scale spontaneous self-organization and maturation of skeletal muscle tissues on ultra-compliant gelatin hydrogel substrates.

Scientific reports

Jensen JH,Cakal SD,Li J,Pless CJ,Radeke C,Jepsen ML,Jensen TE,Dufva M,Lind JU

MA1-22863 was used in Immunocytochemistry-immunoflourescence to show that C2C12 myoblasts spontaneously organize into highly aligned myotube tissues on the mm to cm scale, when cultured on sufficiently soft yet fully isotropic gelatin hydrogel substrates.

Thu Aug 06 00:00:00 UTC 2020

Large-scale spontaneous self-organization and maturation of skeletal muscle tissues on ultra-compliant gelatin hydrogel substrates.

Scientific reports

Jensen JH,Cakal SD,Li J,Pless CJ,Radeke C,Jepsen ML,Jensen TE,Dufva M,Lind JU

Thu Aug 06 00:00:00 UTC 2020

Human Cardiac Organoids for Modeling Genetic Cardiomyopathy.

Cells

Filippo Buono M,von Boehmer L,Strang J,Hoerstrup SP,Emmert MY,Nugraha B

MA1-22863 was used in Immunocytochemistry to demonstrate the generation of cardiac organoids using a triculture approach of human induced pluripotent stem-cell-derived cardiomyocytes (hiPS-CMs)- from healthy subjects and cardiomyopathy patients-human cardiac microvascular endothelial cells (HCMECs) and human cardiac fibroblasts (HCFs).

Mon Jul 20 00:00:00 UTC 2020

On-site fabrication of Bi-layered adhesive mesenchymal stromal cell-dressings for the treatment of heart failure.

Biomaterials

Kobayashi K,Ichihara Y,Sato N,Umeda N,Fields L,Fukumitsu M,Tago Y,Ito T,Kainuma S,Podaru M,Lewis-McDougall F,Yamahara K,Uppal R,Suzuki K

MA1-22863 was used in Immunohistochemistry to address the issue of poor donor cell engraftment following current mesenchymal stromal/stem cell delivery methods.

Mon Jul 01 00:00:00 UTC 2019

Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes.

eLife

Selvaraj S,Mondragon-Gonzalez R,Xu B,Magli A,Kim H,Lainé J,Kiley J,Mckee H,Rinaldi F,Aho J,Tabti N,Shen W,Perlingeiro RC

MA1-22863 was used in Immunocytochemistry to investigate pluripotent stem cells differentiation into myotubes in skeletal muscle disease.

Mon Nov 11 00:00:00 UTC 2019

Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis.

Human molecular genetics

Yang C,Xu Y,Yu M,Lee D,Alharti S,Hellen N,Ahmad Shaik N,Banaganapalli B,Sheikh Ali Mohamoud H,Elango R,Przyborski S,Tenin G,Williams S,O'Sullivan J,Al-Radi OO,Atta J,Harding SE,Keavney B,Lako M,Armstrong L

Published figure using alpha Sarcomeric Actinin monoclonal antibody (Product # MA1-22863) in Immunocytochemistry

Tue Aug 15 00:00:00 UTC 2017

From the Cover: High-Throughput Imaging of Cardiac Microtissues for the Assessment of Cardiac Contraction during Drug Discovery.

Toxicological sciences : an official journal of the Society of Toxicology

Pointon A,Pilling J,Dorval T,Wang Y,Archer C,Pollard C

MA1-22863 was used in Immunocytochemistry, Immunohistochemistry - Free Floating, Immunohistochemistry (Paraffin) to report that cardiac microtissues provide a high-throughput experimental model that is both able to detect changes in cardiac contraction with a sensitivity and specificity of 80 and 91%, respectively, and provide insight into the direction of the inotropic response.

Wed Feb 01 00:00:00 UTC 2017

An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish.

eLife

Zou J,Tran D,Baalbaki M,Tang LF,Poon A,Pelonero A,Titus EW,Yuan C,Shi C,Patchava S,Halper E,Garg J,Movsesyan I,Yin C,Wu R,Wilsbacher LD,Liu J,Hager RL,Coughlin SR,Jinek M,Pullinger CR,Kane JP,Hart DO,Kwok PY,Deo RC

MA1-22863 was used in immunohistochemistry to characterize zebrafish with Titin truncations

Fri Oct 16 00:00:00 UTC 2015

Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.

Nature communications

Sharma P,Abbasi C,Lazic S,Teng ACT,Wang D,Dubois N,Ignatchenko V,Wong V,Liu J,Araki T,Tiburcy M,Ackerley C,Zimmermann WH,Hamilton R,Sun Y,Liu PP,Keller G,Stagljar I,Scott IC,Kislinger T,Gramolini AO

MA1-22863 was used in immunocytochemistry to investigate the impact of Tmem65 on heart development

Fri Sep 25 00:00:00 UTC 2015

Cyclin A2 induces cardiac regeneration after myocardial infarction through cytokinesis of adult cardiomyocytes.

Science translational medicine

Shapiro SD,Ranjan AK,Kawase Y,Cheng RK,Kara RJ,Bhattacharya R,Guzman-Martinez G,Sanz J,Garcia MJ,Chaudhry HW

MA1-22863 was used in immunocytochemistry and immunohistochemistry to study the role of adult cardiomyocyte cytokinesis in the mechanism by which adenoviral delivery of cyclin A2 to infarcted porcine heart promotes regeneration

Wed Feb 19 00:00:00 UTC 2014

Dystrophin-deficient cardiomyocytes derived from human urine: new biologic reagents for drug discovery.

Stem cell research

Guan X,Mack DL,Moreno CM,Strande JL,Mathieu J,Shi Y,Markert CD,Wang Z,Liu G,Lawlor MW,Moorefield EC,Jones TN,Fugate JA,Furth ME,Murry CE,Ruohola-Baker H,Zhang Y,Santana LF,Childers MK

MA1-22863 was used in immunohistochemistry to study the generation of cardiomyocytes from human urine-derived stem cells obtained from a patient with Duchenne muscular dystrophy

Sat Mar 01 00:00:00 UTC 2014

Amniotic fluid stem cells morph into a cardiovascular lineage: analysis of a chemically induced cardiac and vascular commitment.

Drug design, development and therapy

Maioli M,Contini G,Santaniello S,Bandiera P,Pigliaru G,Sanna R,Rinaldi S,Delitala AP,Montella A,Bagella L,Ventura C

MA1-22863 was used in immunocytochemistry to study the ability of a mixture of hyaluronic, butyric and retinoic acids to promote the differentiation of amniotic stem cells towards a cardiovascular lineage

Mon May 05 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: actinin 1 smooth muscle; actinin, alpha 1; alpha actinin 1a; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; F-actin cross-linking protein; Non-muscle alpha-actinin-1

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Gene Aliases: 3110023F10Rik; ACTN1; Actn1a; BDPLT15

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UniProt ID: (Human) P12814, (Mouse) Q7TPR4

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Entrez Gene ID: (Human) 87, (Rabbit) 100341378, (Mouse) 109711, (Zebrafish) 560400

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Function(s)

double-stranded RNA binding integrin binding calcium ion binding protein binding vinculin binding ligand-dependent nuclear receptor transcription coactivator activity protein homodimerization activity ion channel binding actin filament binding actin binding protein domain specific binding metal ion binding

Process(es)

platelet degranulation actin filament organization platelet activation platelet formation platelet morphogenesis regulation of apoptotic process focal adhesion assembly actin filament bundle assembly negative regulation of cellular component movement actin filament network formation actin crosslink formation platelet aggregation regulation of nucleic acid-templated transcription cortical cytoskeleton organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Sarcomeric alpha Actinin Monoclonal Antibody (EA-53)

Sarcomeric alpha Actinin Antibody (MA1-22863) in IHC (P)

Product Details

MA1-22863

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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