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Bethyl Laboratories
Sec3 Polyclonal Antibody
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Sec3 Antibody (A303-364A) in IP
Detection of human Sec3 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-Sec3 antibody (Product # A303-364A) used for IP at 6 µg/mg lysate. Sec3 was also immunoprecipitated by rabbit anti-Sec3 antibody A303-363A, which recognizes an upstream epitope. For blotting immunoprecipitated Sec3, A303-363A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A303-364A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 750 and 800 of Human Exocyst Complex Component Sec3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. Alternatively spliced transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Exocyst complex component 1; Exocyst complex component Sec3; SEC3-like 1
Gene Aliases: BM-012; BM-102; EXOC1; SEC3; SEC3L1; SEC3P
UniProt ID: (Human) Q9NV70
Entrez Gene ID: (Human) 55763
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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