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Senataxin Antibody (A301-105A) in WB
Detection of human Senataxin by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, A-549, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody (Product # A301-105A) (lot A301-105A-4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A301-105A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 2627 and 2677 of Human senataxin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Probable RNA/DNA helicase involved in diverse aspects of RNA metabolism and genomic integrity. Plays a role in transcription regulation by its ability to modulate RNA Polymerase II (Pol II) binding to chromatin and through its interaction with proteins involved in transcription (PubMed:19515850, PubMed:21700224). Contributes to the mRNA splicing efficiency and splice site selection (PubMed:19515850). Required for the resolution of R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site, allowing XRN2 recruitment and XRN2-mediated degradation of the downstream cleaved RNA and hence efficient RNA polymerase II (RNAp II) transcription termination (PubMed:19515850, PubMed:21700224, PubMed:26700805). Required for the 3' transcriptional termination of PER1 and CRY2, thus playing an important role in the circadian rhythm regulation. Involved in DNA double-strand breaks damage response generated by oxidative stress (PubMed:17562789). In association with RRP45, targets the RNA exosome complex to sites of transcription-induced DNA damage (PubMed:24105744). Plays a role in the development and maturation of germ cells: essential for male meiosis, acting at the interface of transcription and meiotic recombination, and in the process of gene silencing during meiotic sex chromosome inactivation (MSCI). May be involved in telomeric stability through the regulation of telomere repeat-containing RNA (TERRA) transcription (PubMed:21112256). Plays a role in neurite outgrowth in hippocampal cells through FGF8-activated signaling pathways. Inhibits retinoic acid-induced apoptosis (PubMed:21576111).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amyotrophic lateral sclerosis 4 protein; Probable helicase senataxin; SEN1 homolog; Senataxin
Gene Aliases: ALS4; AOA2; bA479K20.2; KIAA0625; SCAR1; SETX
UniProt ID: (Human) Q7Z333
Entrez Gene ID: (Human) 23064
MAPK cascade
double-strand break repair
DNA recombination
DNA-templated transcription, termination
termination of RNA polymerase II transcription
mRNA splice site selection
RNA processing
cellular response to DNA damage stimulus
spermatogenesis
circadian rhythm
fibroblast growth factor receptor signaling pathway
positive regulation of neuron projection development
DNA duplex unwinding
positive regulation of RNA splicing
cellular response to oxidative stress
negative regulation of apoptotic process
protein kinase B signaling
cellular response to fibroblast growth factor stimulus
positive regulation of transcription from RNA polymerase II promoter
positive regulation of DNA-templated transcription, termination
cellular response to hydrogen peroxide
cellular response to retinoic acid
positive regulation of DNA-templated transcription, initiation
positive regulation of termination of RNA polymerase II transcription, poly(A)-coupled
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