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Septin 3 Antibody (PA5-144067) in ICC/IF
Immunocytochemistry analysis of Septin 3 in SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Septin 3 Polyclonal antibody (Product # PA5-144067) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropr... View More
Product Details
PA5-144067
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Septin 3/SEPTIN3 recombinant protein (Position: M1-E339).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075281
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Daudi whole cell, human SiHa whole cell, rat brain tissue, mouse brain tissue. IHC: mouse brain tissue, rat brain tissue. ICC/IF: SiHa cell. Flow: U251 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-Sep; CTA-250D10.3; G-septin; G-septin alpha; MGC133218; neuronal-specific septin 3; Neuronal-specific septin-3; OTTHUMP00000028742; P40
Gene Aliases: 3110018K01Rik; AV154067; B530002E20Rik; bK250D10.3; SEP3; SEPT3; SEPTIN3
UniProt ID: (Human) Q9UH03, (Mouse) Q9Z1S5, (Rat) Q9WU34
Entrez Gene ID: (Human) 55964, (Mouse) 24050, (Rat) 56003
Performance Guarantee
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