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Slo2.2 Antibody (MA5-45434) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Slo2.2 monoclonal antibody (Product # MA5-45434) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Slo2.2 antibody (D) Composite.
Product Details
MA5-45434
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N3/26
Immunogen
Fusion protein amino acids 1168-1237 of rat Slo2.2 (Slack)
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931888
Product Specific Information
1 µg/mL of MA5-45434 was sufficient for detection of Slo2.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 140kDa. Weak human detection.approximately Does not cross-react with KCNT2/Slo2.1/Slick.
Target Information
Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a sodium-activated potassium channel subunit which is thought to function in ion conductance and developmental signaling pathways. Mutations in this gene cause the early-onset epileptic disorders, malignant migrating partial seizures of infancy and autosomal dominant nocturnal frontal lobe epilepsy. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: KCa4.1; KCa4.1KIAA1422FLJ41282; KNa1.1; Potassium channel subfamily T member 1; potassium channel subunit (Slack); potassium channel, sodium activated subfamily T, member 1; potassium channel, subfamily T, member 1; Sequence like a calcium-activated K+ channel; Sequence like a calcium-activated potassium channel subunit; Slo2.2; Sodium and chloride-activated ATP-sensitive potassium channel Slo2.2
Gene Aliases: bA100C15.2; C030030G16Rik; EIEE14; ENFL5; KCa4.1; KCNT1; KIAA1422; rSlo2; SLACK; slo2; Slo2.2
UniProt ID: (Human) Q5JUK3, (Mouse) Q6ZPR4, (Rat) Q9Z258
Entrez Gene ID: (Human) 57582, (Mouse) 227632, (Rat) 60444
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