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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Sodium/Calcium Exchanger Antibodies

          Invitrogen

          Sodium/Calcium Exchanger Monoclonal Antibody (C2C12)

          Advanced Verification
          27 Published Figures
          88 References
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Sodium/Calcium Exchanger Monoclonal Antibody (C2C12)

          • Antibody Testing Data (6)
          • Published Figures (27)
          • Advanced Verification (1)
          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 34

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          Sodium/Calcium Exchanger Antibody (MA3-926) in ICC/IF

          Immunofluorescence analysis of Sodium/Calcium Exchanger was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Sodium/Calcium Exchanger Monoclonal Antibody (C2C12) (Product # MA3-926) at 1:200 dilution ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Sodium/Calcium Exchanger Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Sodium/Calcium Exchanger Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Sodium/Calcium Exchanger Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Sodium/Calcium Exchanger Antibody in Immunohistochemistry (IHC)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody

          Product Details

          MA3-926

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 67 publications 67 publications

          Immunohistochemistry (IHC)

          -
          View 6 publications 6 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1/100
          -

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          1:200
          View 12 publications 12 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 1 publication 1 publication

          in situ PLA (PLA)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Dog, Guinea pig, Human, Mouse, Rabbit, Rat

          Published species

          Bovine, Dog, Guinea pig, Human, Marsupial, Mouse, Pig, Rabbit, Rat, Rodent

          Host/Isotype

          Mouse / IgM

          Class

          Monoclonal

          Type

          Antibody

          Clone

          C2C12

          Immunogen

          Purified canine cardiac sodium/calcium exchanger.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography - MBP

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2191111

          Product Specific Information

          MA3-926 detects sodium/calcium exchanger from human, canine, rabbit, mouse, guinea pig and rat kidney and cardiac tissues.

          MA3-926 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 120 kDa protein representing the sodium/calcium exchanger from guinea pig cardiac extract. The bands seen at 70 kDa and 160 kDa represent a proteolytic fragment and non-reduced exchanger respectively. MA3-926 is not recommended for Western blot procedures of rat tissues. Immunohistochemical staining of the sodium/calcium exchanger in rat heart with MA3-926 results in staining of the plasma membrane and intense staining of cardiac T-tubular membrane.

          The MA3-926 antigen is purified canine cardiac sodium/calcium exchanger. This antibody recognizes an epitope between amino acids 371-525, which is on the intracellular side of the plasma membrane.

          Target Information

          Ca2+ plays a critical role in intracellular signaling and thus regulation of calcium balance is critical for the maintenance of this signaling function. The plasma membrane Na+/Ca2+ (NaCX) exchangers mediate Na+-dependent Ca2+ efflux in a wide variety of cell types. NaCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In mammals, at least 5 distinct genes code for the exchangers: Three NaCX (NaCX-1, NaCX-2, and NaCX-3), and two in the NaCKX family (NaCKX-1 and NaCKX-2). NaCX-1 is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling (Matsuoka et al., 1993). NACX-1 may also be a target for protection of the heart and brain from ischemia and reperfusion injury (Hagihara et al., 2005; Bano et al., 2005; Choi, 2005).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cardiac sarcolemmal sodium-calcium exchanger; Na(+)/Ca(2+)-exchange protein 1; Na+/C2+ exchanger; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; Na-Ca exchanger; Na/Ca exchanger; NKX; sodium-calcium exchanger; sodium/calcium exchanger; Sodium/calcium exchanger 1; sodium/calcium exchanger isoform NaCa13; sodium/calcium exchanger isoform NaCa3; sodium/calcium exchanger isoform NaCa7; sodium/calcium exchanger isoform NaCa9; solute carrier family 8 (sodium/calcium exchanger), member 1; Solute carrier family 8 member 1; solute carrier family 8, member 1

          View more View less

          Gene Aliases: AI852629; AV344025; CNC; D930008O12Rik; Ncx; NCX1; SLC8A1

          View more View less

          UniProt ID: (Dog) P23685, (Human) P32418, (Mouse) P70414, (Rat) Q01728, (Guinea pig) P48766

          View more View less

          Entrez Gene ID: (Dog) 475738, (Rabbit) 100328570, (Human) 6546, (Mouse) 20541, (Rat) 29715, (Guinea pig) 100135620

          View more View less

          Function(s)
          calcium:sodium antiporter activity calcium ion binding calmodulin binding cytoskeletal protein binding protein binding ankyrin binding ion channel binding antiporter activity metal ion binding calcium:sodium antiporter activity involved in regulation of cardiac muscle cell membrane potential
          Process(es)
          embryonic placenta development heart morphogenesis cellular sodium ion homeostasis cell communication post-embryonic development positive regulation of bone mineralization cellular response to reactive oxygen species embryonic heart tube development sodium ion transmembrane transport response to muscle stretch muscle fiber development cardiac muscle cell development cardiac muscle contraction calcium ion import calcium ion transmembrane transport sodium ion export sodium ion import positive regulation of the force of heart contraction calcium ion export response to hypoxia regulation of the force of heart contraction regulation of heart rate regulation of sodium ion transport transport ion transport sodium ion transport calcium ion transport cellular calcium ion homeostasis positive regulation of cytosolic calcium ion concentration positive regulation of fibroblast migration regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion vascular smooth muscle contraction response to ATP relaxation of smooth muscle negative regulation of cytosolic calcium ion concentration regulation of calcium ion transport calcium ion homeostasis transmembrane transport calcium ion transport into cytosol cellular response to caffeine cellular response to cAMP regulation of cardiac muscle cell membrane potential cell communication by electrical coupling involved in cardiac conduction response to nutrient response to glucose telencephalon development response to immobilization stress response to drug response to hydrogen peroxide cellular response to hypoxia muscle contraction regulation of cell communication by electrical coupling regulation of cardiac muscle contraction by calcium ion signaling relaxation of cardiac muscle cytosolic calcium ion transport membrane depolarization during cardiac muscle cell action potential regulation of cardiac conduction
          It has to be done as per old AB suggested Products section.
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