Invitrogen

Somatostatin Monoclonal Antibody (ICDCLS), eBioscience™

2 Published Figures

6 References

View all (23) Somatostatin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

Somatostatin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

Somatostatin Antibody (14-9751-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human pancreas tissue using 0.1 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 0.1 µg/mL of Anti-Somatostatin Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.

Somatostatin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

14-9751-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.1 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fish, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ICDCLS

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572982

Product Specific Information

Description: The monoclonal antibody ICDCLS recognizes human, mouse, and rat Somatostatin. Somatostatin, also known as growth hormone-inhibiting hormone (GHIH) or somatotrophin release-inhibiting factor (SRIF), is a peptide hormone with two active forms (14 and 28 amino acids) produced by alternative cleavage of a single preproprotein. Somatostatin is secreted by the delta cells of the islet of Langerhans in the pancreas as well as by the hypothalamus and intestine. Somatostatin has a variety of functions, such as regulating the endocrine system and affecting neurotransmission and cell proliferation. Somatostatin also inhibits the release of numerous secondary hormones, such as insulin and glucagon. Somatostatin can be used to identify medium size aspiny neurons in the striatum, cortex, and hippocampus. Expression of somatostatin is present in hyperplasia of the pancreatic islets as well as islet cell tumors such as somatostatinomas.

Applications Reported: This ICDCLS antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.

Applications Tested: This ICDCLS antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PDX1^LOW MAFA^LOW β-cells contribute to islet function and insulin release.

Nature communications

Nasteska D,Fine NHF,Ashford FB,Cuozzo F,Viloria K,Smith G,Dahir A,Dawson PWJ,Lai YC,Bastidas-Ponce A,Bakhti M,Rutter GA,Fiancette R,Nano R,Piemonti L,Lickert H,Zhou Q,Akerman I,Hodson DJ

14-9751 was used in Immunohistochemistry-immunofluorescence to show that subtle differences in β-cell maturity, defined using PDX1 and MAFA expression, contribute to islet operation.

Fri Jan 29 00:00:00 UTC 2021

Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons.

eLife

Tereshko L,Gao Y,Cary BA,Turrigiano GG,Sengupta P

14-9751-82 was used in Immunocytochemistry to indicate that ciliary neuropeptidergic signaling dynamically modulates excitatory synapses and suggest that defects in this regulation may underlie a subset of behavioral and cognitive disorders associated with ciliopathies.

Tue Mar 02 00:00:00 UTC 2021

Vitamin-D-Binding Protein Contributes to the Maintenance of α Cell Function and Glucagon Secretion.

Cell reports

Viloria K,Nasteska D,Briant LJB,Heising S,Larner DP,Fine NHF,Ashford FB,da Silva Xavier G,Ramos MJ,Hasib A,Cuozzo F,Manning Fox JE,MacDonald PE,Akerman I,Lavery GG,Flaxman C,Morgan NG,Richardson SJ,Hewison M,Hodson DJ

14-9751 was used in Immunohistochemistry to show that vitamin-D-binding protein (DBP) regulates α cell morphology, α cell function, and glucagon secretion.

Tue Jun 16 00:00:00 UTC 2020

Vitamin-D-Binding Protein Contributes to the Maintenance of α Cell Function and Glucagon Secretion.

Cell reports

14-9751 was used in Immunohistochemistry to show that vitamin-D-binding protein (DBP) regulates α cell morphology, α cell function, and glucagon secretion.

Tue Jun 16 00:00:00 UTC 2020

Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics.

Nature communications

Ast J,Arvaniti A,Fine NHF,Nasteska D,Ashford FB,Stamataki Z,Koszegi Z,Bacon A,Jones BJ,Lucey MA,Sasaki S,Brierley DI,Hastoy B,Tomas A,D'Agostino G,Reimann F,Lynn FC,Reissaus CA,Linnemann AK,D'Este E,Calebiro D,Trapp S,Johnsson K,Podewin T,Broichhagen J,Hodson DJ

Published figure using Somatostatin monoclonal antibody (Product # 14-9751-82) in Immunohistochemistry

Fri Jan 24 00:00:00 UTC 2020

Immunohistochemical distribution of insulin-, glucagon- and somatostatin-containing cells in the pancreas of Lake Van fish (Alburnus tarichi Güldenstädt, 1814) (Cyprinidae).

European journal of histochemistry : EJH

Kaptaner B

14-9751-82 was used in Immunohistochemistry (Paraffin) to investigate the existence, regional distribution, and relative frequency of the insulin-, glucagon- and somatostatin-IR cells in the pancreatic tissue of Lake Van fish.

Mon Feb 25 00:00:00 UTC 2019

The Expression of Aldolase B in Islets Is Negatively Associated With Insulin Secretion in Humans.

The Journal of clinical endocrinology and metabolism

Gerst F,Jaghutriz BA,Staiger H,Schulte AM,Lorza-Gil E,Kaiser G,Panse M,Haug S,Heni M,Schütz M,Stadion M,Schürmann A,Marzetta F,Ibberson M,Sipos B,Fend F,Fleming T,Nawroth PP,Königsrainer A,Nadalin S,Wagner S,Peter A,Fritsche A,Richter D,Solimena M,Häring HU,Ullrich S,Wagner R

14-9751-82 was used in Immunohistochemistry to investigate alterations of islet gene expression and corresponding gene variants in the context of in vivo glycemic traits from the same patients.

Sat Dec 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: Growth hormone release-inhibiting factor; prepro-somatostatin; preprosomatostatin; Somatostatin; somatostatin 28; somatostatin-14; somatostatin-28; somatotropin release-inhibiting factor; SRIF

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Gene Aliases: SMST; SOM; SRIF; SS; SS-14; SS-28; SST

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UniProt ID: (Human) P61278, (Mouse) P60041, (Rat) P60042

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Entrez Gene ID: (Human) 6750, (Mouse) 20604, (Rat) 24797

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Function(s)

hormone activity

Process(es)

hyperosmotic response cell surface receptor signaling pathway G-protein coupled receptor signaling pathway cell-cell signaling chemical synaptic transmission response to nutrient digestion negative regulation of cell proliferation hormone-mediated apoptotic signaling pathway response to heat response to acidic pH regulation of cell migration response to drug response to amino acid response to steroid hormone response to organonitrogen compound

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Somatostatin Monoclonal Antibody (ICDCLS), eBioscience™

Somatostatin Antibody (14-9751-82) in IHC (P)

Product Details

14-9751-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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