Proteintech

Spartin/SPG20 Polyclonal Antibody

Spartin/SPG20 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

Spartin/SPG20 Antibody (13791-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using SPG20 antibody 13791-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Spartin/SPG20 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Spartin/SPG20 Antibody in Western Blot (WB)

Spartin/SPG20 Antibody in Flow Cytometry (Flow)

Spartin/SPG20 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13791-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Spartin, SPG20 Fusion Protein Ag4815 (1-350 aa encoded by BC047083)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEQEPQNGE PAEIKIIREA YKKAFLFVNK GLNTDELGQK EEAKNYYKQG IGHLLRGISI SSKESEHTGT GWESARQMQQ KMKETLQNVR TRLEILEKGL ATSLQNDLQE VPKLYPEFPP KDMCEKLPEP QSFSSAPQHA EVNGNTSTPS AGAVAAPASL SLPSQSCPAE APPAYTPQAA EGHYTVSYGT DSGEFSSVGE EFYRNHSQPP PLETLGLDAD ELILIPNGVQ IFFVNPAGEV SAPSYPGYLR IVRFLDNSLD TVLNRPPGFL QVCDWLYPLV PDRSPVLKCT AGAYMFPDTM LQAAGCFVGV VLSSELPEDD RELFEDLLRQ MSDLRLQANW NRAEEENEFQ I (1-350 aa encoded by BC047083)

Target Information

SPG20 is a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2.

Nature communications

Nalbach K,Schifferer M,Bhattacharya D,Ho-Xuan H,Tseng WC,Williams LA,Stolz A,Lichtenthaler SF,Elazar Z,Behrends C

Thu Feb 16 00:00:00 UTC 2023

A novel mutation in SPART gene causes a severe neurodevelopmental delay due to mitochondrial dysfunction with complex I impairments and altered pyruvate metabolism.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Diquigiovanni C,Bergamini C,Diaz R,Liparulo I,Bianco F,Masin L,Baldassarro VA,Rizzardi N,Tranchina A,Buscherini F,Wischmeijer A,Pippucci T,Scarano E,Cordelli DM,Fato R,Seri M,Paracchini S,Bonora E

Tue Oct 01 00:00:00 UTC 2019

A novel mutation in SPART gene causes a severe neurodevelopmental delay due to mitochondrial dysfunction with complex I impairments and altered pyruvate metabolism.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Diquigiovanni C,Bergamini C,Diaz R,Liparulo I,Bianco F,Masin L,Baldassarro VA,Rizzardi N,Tranchina A,Buscherini F,Wischmeijer A,Pippucci T,Scarano E,Cordelli DM,Fato R,Seri M,Paracchini S,Bonora E

Tue Oct 01 00:00:00 UTC 2019

Novel Homozygous Missense Mutation in SPG20 Gene Results in Troyer Syndrome Associated with Mitochondrial Cytochrome c Oxidase Deficiency.

JIMD reports

Spiegel R,Soiferman D,Shaag A,Shalev S,Elpeleg O,Saada A

Wed Sep 30 00:00:00 UTC 2020

Novel Homozygous Missense Mutation in SPG20 Gene Results in Troyer Syndrome Associated with Mitochondrial Cytochrome c Oxidase Deficiency.

JIMD reports

Spiegel R,Soiferman D,Shaag A,Shalev S,Elpeleg O,Saada A

Wed Sep 30 00:00:00 UTC 2020

Germline deletion of huntingtin causes male infertility and arrested spermiogenesis in mice.

Journal of cell science

Yan J,Zhang H,Liu Y,Zhao F,Zhu S,Xie C,Tang TS,Guo C

Mon Feb 01 00:00:00 UTC 2016

Germline deletion of huntingtin causes male infertility and arrested spermiogenesis in mice.

Journal of cell science

Yan J,Zhang H,Liu Y,Zhao F,Zhu S,Xie C,Tang TS,Guo C

Mon Feb 01 00:00:00 UTC 2016

Chromosome mis-segregation and cytokinesis failure in trisomic human cells.

eLife

Nicholson JM,Macedo JC,Mattingly AJ,Wangsa D,Camps J,Lima V,Gomes AM,Dória S,Ried T,Logarinho E,Cimini D

Tue May 05 00:00:00 UTC 2015

Chromosome mis-segregation and cytokinesis failure in trisomic human cells.

eLife

Nicholson JM,Macedo JC,Mattingly AJ,Wangsa D,Camps J,Lima V,Gomes AM,Dória S,Ried T,Logarinho E,Cimini D

Tue May 05 00:00:00 UTC 2015

Different expression levels of spartin cause broad spectrum of cellular consequences in human neuroblastoma cells.

Cell biology international

Milewska M,Byrne PC

Tue Sep 01 00:00:00 UTC 2015

Different expression levels of spartin cause broad spectrum of cellular consequences in human neuroblastoma cells.

Cell biology international

Milewska M,Byrne PC

Tue Sep 01 00:00:00 UTC 2015

The role of spartin and its novel ubiquitin binding region in DALIS occurrence.

Molecular biology of the cell

Karlsson AB,Washington J,Dimitrova V,Hooper C,Shekhtman A,Bakowska JC

Tue Apr 01 00:00:00 UTC 2014

The role of spartin and its novel ubiquitin binding region in DALIS occurrence.

Molecular biology of the cell

Karlsson AB,Washington J,Dimitrova V,Hooper C,Shekhtman A,Bakowska JC

Tue Apr 01 00:00:00 UTC 2014

The role of spartin and its novel ubiquitin binding region in DALIS occurrence.

Molecular biology of the cell

Karlsson AB,Washington J,Dimitrova V,Hooper C,Shekhtman A,Bakowska JC

Tue Apr 01 00:00:00 UTC 2014

The role of spartin and its novel ubiquitin binding region in DALIS occurrence.

Molecular biology of the cell

Karlsson AB,Washington J,Dimitrova V,Hooper C,Shekhtman A,Bakowska JC

Tue Apr 01 00:00:00 UTC 2014

Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling.

Human molecular genetics

Renvoisé B,Stadler J,Singh R,Bakowska JC,Blackstone C

Wed Aug 15 00:00:00 UTC 2012

Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling.

Human molecular genetics

Renvoisé B,Stadler J,Singh R,Bakowska JC,Blackstone C

Wed Aug 15 00:00:00 UTC 2012

Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling.

Human molecular genetics

Renvoisé B,Stadler J,Singh R,Bakowska JC,Blackstone C

Wed Aug 15 00:00:00 UTC 2012

Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling.

Human molecular genetics

Renvoisé B,Stadler J,Singh R,Bakowska JC,Blackstone C

Wed Aug 15 00:00:00 UTC 2012

SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis.

Oncogene

Lind GE,Raiborg C,Danielsen SA,Rognum TO,Thiis-Evensen E,Hoff G,Nesbakken A,Stenmark H,Lothe RA

Thu Sep 15 00:00:00 UTC 2011

SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis.

Oncogene

Lind GE,Raiborg C,Danielsen SA,Rognum TO,Thiis-Evensen E,Hoff G,Nesbakken A,Stenmark H,Lothe RA

Thu Sep 15 00:00:00 UTC 2011

SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis.

Oncogene

Lind GE,Raiborg C,Danielsen SA,Rognum TO,Thiis-Evensen E,Hoff G,Nesbakken A,Stenmark H,Lothe RA

Thu Sep 15 00:00:00 UTC 2011

SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis.

Oncogene

Lind GE,Raiborg C,Danielsen SA,Rognum TO,Thiis-Evensen E,Hoff G,Nesbakken A,Stenmark H,Lothe RA

Thu Sep 15 00:00:00 UTC 2011

Spartin recruits PKC-ζ via the PKC-ζ-interacting proteins ZIP1 and ZIP3 to lipid droplets.

Journal of neurochemistry

Urbanczyk A,Enz R

Thu Sep 01 00:00:00 UTC 2011

Spartin recruits PKC-ζ via the PKC-ζ-interacting proteins ZIP1 and ZIP3 to lipid droplets.

Journal of neurochemistry

Urbanczyk A,Enz R

Thu Sep 01 00:00:00 UTC 2011

Spartin recruits PKC-ζ via the PKC-ζ-interacting proteins ZIP1 and ZIP3 to lipid droplets.

Journal of neurochemistry

Urbanczyk A,Enz R

Thu Sep 01 00:00:00 UTC 2011

Developmental and degenerative features in a complicated spastic paraplegia.

Annals of neurology

Manzini MC,Rajab A,Maynard TM,Mochida GH,Tan WH,Nasir R,Hill RS,Gleason D,Al Saffar M,Partlow JN,Barry BJ,Vernon M,LaMantia AS,Walsh CA

Thu Apr 01 00:00:00 UTC 2010

Developmental and degenerative features in a complicated spastic paraplegia.

Annals of neurology

Manzini MC,Rajab A,Maynard TM,Mochida GH,Tan WH,Nasir R,Hill RS,Gleason D,Al Saffar M,Partlow JN,Barry BJ,Vernon M,LaMantia AS,Walsh CA

Thu Apr 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: Spartin; spastic paraplegia 20 (Troyer syndrome) homolog; Spastic paraplegia 20 protein; spastic paraplegia 20, spartin (Troyer syndrome) homolog; Trans-activated by hepatitis C virus core protein 1

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Gene Aliases: AI840044; C79168; KIAA0610; mKIAA0610; SPART; SPARTIN; SPG20; TAHCCP1

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UniProt ID: (Human) Q8N0X7, (Mouse) Q8R1X6

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Entrez Gene ID: (Human) 23111, (Rat) 295053, (Mouse) 229285

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Function(s)

protein binding ubiquitin protein ligase binding

Process(es)

abscission negative regulation of BMP signaling pathway lipid particle organization negative regulation of collateral sprouting in absence of injury neuromuscular process cell division regulation of mitochondrial membrane potential adipose tissue development cytokinesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Spartin/SPG20 Polyclonal Antibody

Spartin/SPG20 Antibody (13791-1-AP) in ICC/IF

Product Details

13791-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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