Invitrogen

Spectrin beta-3 Polyclonal Antibody

1 Published Figure

5 References

View all (29) SPTBN2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

Spectrin beta-3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 2

Spectrin beta-3 Antibody (PA1-46007) in IHC (P)

Immunohistochemical analysis of Spectrin beta-3 in human prostate epithelium. Samples were incubated in Spectrin beta-3 polyclonal antibody (Product # PA1-46007).

Spectrin beta-3 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

PA1-46007

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

10-20 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

2-5 µg/mg lysate

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The epitope recognized by this antibody maps to a C-terminal region between residue 2340 and 2390 of spectrin beta 3.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2194518

Product Specific Information

Suggested positive control: Hela whole cell extract.

Target Information

Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Innovative Computerized Dystrophin Quantification Method Based on Spectral Confocal Microscopy.

International journal of molecular sciences

Codina A,Roldán M,Natera-de Benito D,Ortez C,Planas R,Matalonga L,Cuadras D,Carrera L,Exposito J,Marquez J,Jimenez-Mallebrera C,M Porta J,Nascimento A,Jou C

PA1-46007 was used in Immunohistochemistry to develop a method for the quantification of dystrophin in DMD and BMD patients using spectral confocal microscopy.

Tue Mar 28 00:00:00 UTC 2023

Large in-frame 5' deletions in DMD associated with mild Duchenne muscular dystrophy: Two case reports and a review of the literature.

Neuromuscular disorders : NMD

Gibbs EM,Barthélémy F,Douine ED,Hardiman NC,Shieh PB,Khanlou N,Crosbie RH,Nelson SF,Miceli MC

PA1-46007 was used in Immunocytochemistry-immunofluorescence to describe two individuals with large in-frame 5' deletions (exon 3-23 and exon 3-28) that remove the majority of the N-terminal region, including part of the actin binding and central rod domains.

Fri Nov 01 00:00:00 UTC 2019

MTSS1/Src family kinase dysregulation underlies multiple inherited ataxias.

Proceedings of the National Academy of Sciences of the United States of America

Brown AS,Meera P,Altindag B,Chopra R,Perkins EM,Paul S,Scoles DR,Tarapore E,Magri J,Huang H,Jackson M,Shakkottai VG,Otis TS,Pulst SM,Atwood SX,Oro AE

PA1-46007 was used in Western Blotting to identify a common SCA therapeutic target and demonstrate a key role for MTSS1/SFK in Purkinje neuron survival and ataxia progression.

Wed Dec 26 00:00:00 UTC 2018

A sensitive, reproducible and objective immunofluorescence analysis method of dystrophin in individual fibers in samples from patients with duchenne muscular dystrophy.

PloS one

Beekman C,Sipkens JA,Testerink J,Giannakopoulos S,Kreuger D,van Deutekom JC,Campion GV,de Kimpe SJ,Lourbakos A

PA1-46007 was used in immunohistochemistry - frozen section to develop an immunofluorescence and semi-automated image analysis method to assess the sarcolemmal dystrophin intensity per individual fiber in a muscle biopsy

Tue Jun 16 00:00:00 UTC 2015

Prednisolone treatment does not interfere with 2'-O-methyl phosphorothioate antisense-mediated exon skipping in Duchenne muscular dystrophy.

Human gene therapy

Verhaart IE,Heemskerk H,Karnaoukh TG,Kolfschoten IG,Vroon A,van Ommen GJ,van Deutekom JC,Aartsma-Rus A

PA1-46007 was used in immunohistochemistry to study the effect of prednisolone treatment on exon skipping in Duchenne muscular dystrophy

Thu Mar 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Beta-III spectrin; beta-spectrin 3; glutamate transporter EAAT4-associated protein 41; Spectrin Beta; spectrin beta 3; spectrin beta chain, brain 2; Spectrin beta chain, non-erythrocytic 2; spectrin beta III sigma 2; spectrin, beta, non-erythrocytic 2; spectrin, non-erythroid beta chain 2; Spinocerebellar ataxia 5 protein

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Gene Aliases: GTRAP41; KIAA0302; mKIAA0302; SCA5; SCAR14; Spnb3; SPTBN2

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UniProt ID: (Human) O15020

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Entrez Gene ID: (Human) 6712, (Mouse) 20743

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Function(s)

actin binding Ras guanyl-nucleotide exchange factor activity structural constituent of cytoskeleton phospholipid binding cadherin binding involved in cell-cell adhesion

Process(es)

synapse assembly synaptic vesicle exocytosis vesicle-mediated transport cerebellar Purkinje cell layer morphogenesis adult behavior multicellular organism growth MAPK cascade ER to Golgi vesicle-mediated transport cytoskeleton organization axon guidance antigen processing and presentation of exogenous peptide antigen via MHC class II positive regulation of GTPase activity actin filament capping cellular response to ethanol cell-cell adhesion

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Spectrin beta-3 Polyclonal Antibody

Spectrin beta-3 Antibody (PA1-46007) in IHC (P)

Product Details

PA1-46007

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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