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Rockland
Sprouty-2 Polyclonal Antibody
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Sprouty-2 Antibody (600-401-E68) in IHC (P)
Immunohistochemistry of rabbit anti-Sprouty-2 antibody. Tissue: kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-sprouty 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.
Product Details
600-401-E68
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Sprouty-2 affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with peptide corresponding to an internal region of human sprouty-2.
Conjugate
Form
Liquid
Concentration
1.04 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Sprouty-2 antibody reacts with human Sprouty 2 protein. A BLAST analysis was used to suggest reactivity with Sprouty 2 from human, monkey, and orangutan based on a 100% homology with the immunizing sequence. Cross-reactivity with Sprouty2 from other sources has not been determined.
Target Information
This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC23039; Protein sprouty homolog 2; Spry-2
Gene Aliases: hSPRY2; IGAN3; SPRY2
UniProt ID: (Human) O43597
Entrez Gene ID: (Human) 10253
establishment of mitotic spindle orientation
sensory perception of sound
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of peptidyl-threonine phosphorylation
negative regulation of cell projection organization
positive regulation of peptidyl-serine phosphorylation
negative regulation of GTPase activity
negative regulation of fibroblast growth factor receptor signaling pathway
negative regulation of epidermal growth factor receptor signaling pathway
inner ear morphogenesis
negative regulation of apoptotic process
negative regulation of MAP kinase activity
cell fate commitment
regulation of cell differentiation
negative regulation of Ras protein signal transduction
negative regulation of neurotrophin TRK receptor signaling pathway
positive regulation of protein kinase B signaling
lung growth
bud elongation involved in lung branching
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of protein serine/threonine kinase activity
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