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Sprouty-4 Antibody (600-401-697) in IHC (P)
Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated red signal with he... View More
Product Details
600-401-697
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chimpanzee,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human Sprouty-4 protein.
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human Sprouty-4 protein. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity is expected with Sprouty-4.
Target Information
SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. The protein is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein sprouty homolog 4; Sprouty 4; sprouty homolog 4; Spry-4
Gene Aliases: HH17; SPRY4
UniProt ID: (Human) Q9C004
Entrez Gene ID: (Human) 81848
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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