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FIGURE: 1 / 1
Stella Antibody (711460) in ICC/IF
For immunofluorescence analysis, NCCIT cells were fixed and permeabilized for detection of endogenous DPPa3 using DPPa3 Recombinant Rabbit Superclonal™ Antibody (Product # 711460, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of DPPa3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938).... View More
Product Details
711460
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
17HCLC
Immunogen
Protein corresponding to Human DPPA3 (aa 1-159)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633182
Product Specific Information
This antibody is predicted to react with Monkey, Rat and Horse.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
DPPa3 (stella-related protein) is a stem cell marker protein which is restricted to pluripotent human embryonic stem cells, premeiotic germ lineage cells and testicular germ cell tumor cells. Otherwise known as 'developmental pluripotency-associated protein 3,' it is expressed in primordial germ cells, Oocytes, preimplantation embryos and pluripotent cells. This protein has a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Developmental pluripotency-associated protein 3; Stella-related protein
Gene Aliases: DPPA3; STELLA; STELLAR
UniProt ID: (Human) Q6W0C5
Entrez Gene ID: (Human) 359787
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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