Invitrogen

Strep Tag Monoclonal Antibody (GT661)

1 Published Figure

6 References

View all (9) Strep Tag antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (1)

Strep Tag Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

Strep Tag Antibody (MA5-17283) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Strep Tag in Strep/GFP tag transfected HeLa cells (fixed with 4% paraformaldehyde at RT for 15 min) using Strep Tag Monoclonal Antibody (GT661) (Product # MA5 17283) diluted at 1:500 (Green: GFP is expressed in the tranfected cell). Blue: Hoechst 33342 staining.

Strep Tag Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

MA5-17283

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GT661

Immunogen

Amino acid sequence - ASWSHPQFEKGA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2538749

Product Specific Information

Recommended positive controls: strep-tagged protein expressing 293T cells.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia.

Redox biology

Archer SL,Dasgupta A,Chen KH,Wu D,Baid K,Mamatis JE,Gonzalez V,Read A,Bentley RE,Martin AY,Mewburn JD,Dunham-Snary KJ,Evans GA,Levy G,Jones O,Al-Qazazi R,Ring B,Alizadeh E,Hindmarch CC,Rossi J,Lima PD,Falzarano D,Banerjee A,Colpitts CC

MA5-17283 was used in Western Blot to assess HPV via cardiac catheterization and apoptosis using the TUNEL assay.

Thu Dec 01 00:00:00 UTC 2022

Microbial Protein Binding to gC1qR Drives PLA2G1B-Induced CD4 T-Cell Anergy.

Frontiers in immunology

Pothlichet J,Meola A,Bugault F,Jeammet L,Savitt AG,Ghebrehiwet B,Touqui L,Pouletty P,Fiore F,Sauvanet A,Thèze J

MA5-17283 was used in ELISA to suggest that the PLA2G1B/cofactor system is a general CD4 T-cell inhibitor and pave the way for further studies to better understand the role of CD4 T-cell anergy in infectious diseases and tumor escape.

Mon Apr 11 00:00:00 UTC 2022

Enterovirus 3C Protease Cleaves TRIM7 To Dampen Its Antiviral Activity.

Journal of virology

Fan W,McDougal MB,Schoggins JW

MA5-17283 was used in Western Blot to express protein variants with distinct antiviral activities and susceptibilities to viral protease-mediated cleavage.

Wed Oct 12 00:00:00 UTC 2022

TRIM7 inhibits enterovirus replication and promotes emergence of a viral variant with increased pathogenicity.

Cell

Fan W,Mar KB,Sari L,Gaszek IK,Cheng Q,Evers BM,Shelton JM,Wight-Carter M,Siegwart DJ,Lin MM,Schoggins JW

MA5-17283 was used in Western Blotting to reveal a unique mechanism for targeting enterovirus replication and provide molecular insight into the benefits and trade-offs of viral evolution imposed by a host restriction factor.

Thu Jun 24 00:00:00 UTC 2021

The Human Cytomegalovirus UL116 Glycoprotein Is a Chaperone to Control gH-Based Complexes Levels on Virions.

Frontiers in microbiology

Vezzani G,Amendola D,Yu D,Chandramouli S,Frigimelica E,Maione D,Merola M

MA5-17283 was used in Western Blot to show that results are consistent with a role of UL116 as a chaperone for gH during the assembly and maturation of gH complexes in infected cells.

Sat Apr 24 00:00:00 UTC 2021

The Human Cytomegalovirus UL116 Glycoprotein Is a Chaperone to Control gH-Based Complexes Levels on Virions.

Frontiers in microbiology

Vezzani G,Amendola D,Yu D,Chandramouli S,Frigimelica E,Maione D,Merola M

MA5-17283 was used in Western Blot to show that results are consistent with a role of UL116 as a chaperone for gH during the assembly and maturation of gH complexes in infected cells.

Sat Apr 24 00:00:00 UTC 2021

Structural analysis of RIG-I-like receptors reveals ancient rules of engagement between diverse RNA helicases and TRIM ubiquitin ligases.

Molecular cell

Kato K,Ahmad S,Zhu Z,Young JM,Mu X,Park S,Malik HS,Hur S

MA5-17283 was used in Immunocytochemistry, Immunoprecipitation, Western Blot to uncover evolutionarily conserved rules of engagement between RNA helicases and tripartite motif (TRIM) E3 ligases that lead to their functional coordination in vertebrate innate immunity.

Thu Feb 04 00:00:00 UTC 2021

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Strep Tag Monoclonal Antibody (GT661)

Strep Tag Antibody (MA5-17283) in ICC/IF

Product Details

MA5-17283

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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