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Strep tag Antibody (910STR) in WB
Western blot analysis of E. coli lysate containing Strep-tagged protein with anti-Strep-tag antibody. Western blot analysis was performed on 80 ng, 40 ng, or 10 ng of E. coli lysate containing Strep-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1h at RT. The primary antibody anti-Strep-tag mouse monoclonal, C23.21 (Cat. No. 910STRL) was diluted in blocking buffer (antibody concentration 0.02 µg/mL) and incubated for 1h at RT. The secondary antibody goat anti-mouse IgG po... View More
Product Details
910STR
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
C23.21
Immunogen
Strep-Tag Ii Peptide Minimal Sequence Wshpqfek
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute with 250 µL PBS.
Specificity: Strep II. A Step-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Target Information
Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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