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Invitrogen
Sulfotyrosine Monoclonal Antibody (7C5), PerCP
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FIGURE: 1 / 2
Sulfotyrosine Antibody (MA5-45835) in IHC (P)
Immunohistochemistry analysis using kidney. Fixation involved formalin fixed, paraffin embedded treatment. Samples were incubated with Sulfotyrosine monoclonal antibody (Product # MA5-45835) at 1:25 for 1 hour at RT, followed by Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain used was Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (... View More
Product Details
MA5-45835
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
7C5
Immunogen
Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932289
Product Specific Information
A 1:1,000 dilution of MA5-45835 was sufficient for detection of Sulfotyrosine in 0.5 µg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
Target Information
Sulfotyrosine is involved in protein-protein interactions. Sulfotyrosines are present in all multicellular eukaryotes, but unicellular eukaryotes such as yeast and prokaryotes are not capable of tyrosine sulfation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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