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Invitrogen
Supt4h1 Polyclonal Antibody
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Product Details
PA5-75694
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues in Human SUPT4H1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2719422
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DRB sensitivity-inducing factor 14 kDa subunit; DRB sensitivity-inducing factor small subunit; DRB sensitivity-inducing factor small subunit 1; DSIF p14; DSIF small subunit; DSIF small subunit 1; hSPT4; small hSpt4 subunit; suppressor of Ty 4 homolog 1; suppressor of Ty 4 homolog 2; Transcription elongation factor SPT4; Transcription elongation factor SPT4 1; Transcription elongation factor SPT4-A
Gene Aliases: AL022777; SPT4; SPT4H; Supt4a; SUPT4H; SUPT4H1; Supt4h1a; Supt4h2
UniProt ID: (Human) P63272, (Mouse) P63271
Entrez Gene ID: (Human) 6827, (Mouse) 20922, (Rat) 287608
negative regulation of transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
biological_process
negative regulation of DNA-templated transcription, elongation
positive regulation of DNA-templated transcription, elongation
negative regulation of transcription elongation from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of viral transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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