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Invitrogen
Survivin Monoclonal Antibody (STLALYV), eBioscience™
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FIGURE: 1 / 4
Survivin Antibody (14-9176-82) in ICC/IF
Immunofluorescence analysis of Survivin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Survivin Monoclonal Antibody (STLALYV), eBioscience™ (Product # 14-9176-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor®... View More
Product Details
14-9176-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
STLALYV
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572939
Product Specific Information
Description: This STLALYV monoclonal antibody recognizes human survivin (also known as Birc5). Survivin is a member of the inhibitor of apoptosis (IAP) family that also includes XIAP, c-IAP1, c-IAP2, and NAIP. Survivin functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis. Survivin also plays a critical role in mitosis and cytokinesis where it is expressed only during G2/M and localizes to the mitotic spindle and is found at the cleavage furrow/midplate. Thus, survivin is highly expressed in tumors and in fetal tissue, but is absent in terminally-differentiated cells and non-proliferating cells. Deletion of survivin in developing thymocytes as well as in mature T cells showed that survivin is critical for cell division in lymphoid cells but not for inhibition of survival; the same is true for B lymphocytes.
Applications Reported: This STLALYV antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. (Fluorochrome-conjugated STLALYV is recommended for use in intracellular flow cytometry.).
Applications Tested: This STLALYV antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 5 µg/mL. This STLALYV antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin’s gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apoptosis inhibitor 4; Apoptosis inhibitor survivin; Baculoviral IAP repeat-containing protein 5; BIRC 5; EPR 1; survivin variant 3 alpha; SVV
Gene Aliases: API4; BIRC5; EPR-1; IAP4
UniProt ID: (Human) O15392
Entrez Gene ID: (Human) 332
transcription, DNA-templated
protein phosphorylation
apoptotic process
sister chromatid cohesion
positive regulation of cell proliferation
regulation of signal transduction
protein ubiquitination
protein sumoylation
protein complex localization
regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
cell division
mitotic spindle assembly
inhibition of cysteine-type endopeptidase activity involved in apoptotic process
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