Invitrogen

Syk Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-28574

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Syk. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.02 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2546050

Product Specific Information

Recommended positive controls: A431, Raji.

Predicted reactivity: Mouse (89%), Rat (89%), Pig (90%), Chicken (81%), Rhesus Monkey (91%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72-Syk; p72Syk; SP1; Spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; Tyrosine-protein kinase SYK

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Gene Aliases: p72-Syk; p72syk; ptk72; SYK; Sykb

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UniProt ID: (Human) P43405, (Rat) Q64725, (Mouse) P48025

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Entrez Gene ID: (Human) 6850, (Rat) 25155, (Mouse) 20963

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity integrin binding protein binding ATP binding protein kinase binding receptor binding protein domain specific binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
activation of MAPK activity angiogenesis serotonin secretion lymph vessel development adaptive immune response macrophage activation involved in immune response neutrophil activation involved in immune response leukocyte activation involved in immune response serotonin secretion by platelet protein phosphorylation inflammatory response leukocyte cell-cell adhesion cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway integrin-mediated signaling pathway activation of JUN kinase activity regulation of platelet activation cell migration peptidyl-serine phosphorylation peptidyl-tyrosine phosphorylation leukotriene biosynthetic process signal transduction by protein phosphorylation neutrophil chemotaxis regulation of superoxide anion generation positive regulation of cell adhesion mediated by integrin intracellular signal transduction peptidyl-tyrosine autophosphorylation defense response to bacterium transcription factor import into nucleus positive regulation of mast cell degranulation regulation of neutrophil degranulation beta selection innate immune response positive regulation of interleukin-3 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of bone resorption positive regulation of alpha-beta T cell differentiation positive regulation of alpha-beta T cell proliferation protein autophosphorylation blood vessel morphogenesis positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation regulation of phagocytosis regulation of immune response positive regulation of calcium-mediated signaling B cell receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity regulation of ERK1 and ERK2 cascade cellular response to molecule of fungal origin regulation of arachidonic acid secretion regulation of platelet aggregation positive regulation of receptor internalization stimulatory C-type lectin receptor signaling pathway cell proliferation organ morphogenesis regulation of tumor necrosis factor-mediated signaling pathway viral process platelet activation receptor internalization positive regulation of type I interferon production Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis cellular response to low-density lipoprotein particle stimulus immune system process G-protein coupled receptor signaling pathway phosphorylation cell differentiation
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