Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
TAF10 Monoclonal Antibody (23TA-1H8)
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 3
TAF10 Antibody (MA3-069) in ICC/IF
IImmunofluorescent analysis of TAF10 in HepG2 cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a TAF10 mouse monoclonal antibody (Product # MA3-069) at a dilution of 1:2000 in blocking buffer for 1 hour at room temperature, and then incubated with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:10... View More
Product Details
MA3-069
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
23TA-1H8
Immunogen
Synthetic peptide coupled to ovalalbumin aa 1 to 20
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2637137
Product Specific Information
MA3-069 detects TAF10 from human, rat, and non-human primate samples. MA3-069 does not detect the TAF10 in mouse samples
MA3-069 has been successfully used in Western blot, immunofluorescence and flow cytometry applications.
The MA3-069 immunogen is the synthetic peptide coupled to ovalalbumin aa 1 to aa 20.
Target Information
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: STAF28; TAF(II)30; TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa; TAFII-30; TATA box binding protein (TBP)-associated factor, RNA polymerase II, H, 30kD; TATA box binding protein associated factor 10; transcription initiation factor TFIID 30 kD subunit; Transcription initiation factor TFIID 30 kDa subunit; Transcription initiation factor TFIID subunit 10
Gene Aliases: TAF10; TAF2A; TAF2H; TAFII30
UniProt ID: (Human) Q12962
Entrez Gene ID: (Human) 6881, (Rat) 293345
G1/S transition of mitotic cell cycle
liver development
DNA-templated transcription, initiation
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
apoptotic process
biological_process
regulation of gene expression
histone deubiquitination
multicellular organism growth
cellular protein complex assembly
histone H3 acetylation
regulation of DNA binding
protein homooligomerization
hepatocyte differentiation
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support