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TAF12 Antibody (PA5-106322) in ICC/IF
Immunofluorescent analysis of TAF12 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TAF12 polyclonal antibody (Product # PA5-106322) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-106322
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TAF12(Accession Q16514), corresponding to amino acid residues M1-S51.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2853999
Product Specific Information
Antibody detects endogenous levels of total TAF12.
Target Information
TAF12 is involved in the control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20 kDa; TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa; TAFII-20; TAFII-20/TAFII-15; TAFII20; TAFII20/TAFII15; TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD; TATA box binding protein associated factor 12; Transcription initiation factor TFIID 20 kDa subunits; Transcription initiation factor TFIID 20/15 kDa subunits; Transcription initiation factor TFIID subunit 12
Gene Aliases: 20kDa; 2810422D08Rik; AW557038; TAF12; TAF15; TAF2J; TAFII20
UniProt ID: (Human) Q16514, (Mouse) Q8VE65
Entrez Gene ID: (Human) 6883, (Mouse) 66464, (Rat) 682902
transcription, DNA-templated
DNA-templated transcription, initiation
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
histone H3 acetylation
positive regulation of sequence-specific DNA binding transcription factor activity
regulation of nucleic acid-templated transcription
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
regulation of signal transduction by p53 class mediator
biological_process
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