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Invitrogen
TAF172 Polyclonal Antibody
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TAF172 Antibody (PA5-96434) in WB
Western blot analysis of TAF172 in extracts of various cell lines. Samples were incubated with TAF172 Polyclonal antibody (Product # PA5-96434) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Product Details
PA5-96434
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_0039631)
Conjugate
Form
Liquid
Concentration
0.57 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808236
Product Specific Information
Immunogen sequence: LAVQNSSLHD IQHAPKLSAL KQLLLDCGLG NGSTSESGTE SVVAQHRILI FCQLKSMLDI VEHDLLKPHL PSVTYLRLDG SIPPGQRHSI VSRFNNDPSI DVLLLTTHVG GLGLNLTGAD TVVFVEHDWN PMRDLQAMDR AHRIGQKRVV NVYRLITRGT LEEKIMGLQK FKMNIANTVI SQENSSLQSM GTDQLLDLFT LDKDGKAEKA DTSTSGKASM KSILENLSDL WDQEQYDSEY SLENFMHSLK; Positive Samples: HepG2, HeLa, MCF7, Rat testis; Cellular Location: Nucleus
Target Information
Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP) and TBP-associated factors, or TAFs (e.g., TAF2B), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, (Mot1 homolog, S. cerevisiae); BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae); Mot1 homolog; TAF(II)170; TAF-172; TATA-binding protein-associated factor 172; TBP-associated factor 172
Gene Aliases: BTAF1; MOT1; RGD1564130; TAF(II)170; TAF172; TAFII170
UniProt ID: (Human) O14981
Entrez Gene ID: (Human) 9044, (Rat) 368042
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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