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OriGene
TAF2 Monoclonal Antibody (OTI2A5), TrueMAB™
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FIGURE: 1 / 1
TAF2 Antibody (CF504588) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TAF2 (RC213159, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TAF2. Positive lysates LY418826 (100 µg) and LC418826 (20 µg) can be purchased separately from OriGene.
Product Details
CF504588
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2A5
Immunogen
Human recombinant protein fragment corresponding to amino acids 782-1121 of human TAF2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 150 kDa cofactor of initiator function; cofactor of initiator function, 150kD subunit; RNA polymerase II TBP-associated factor subunit B; TAF(II)150; TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa; TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD; TATA box binding protein associated factor 2; TBP-associated factor 150 kDa; Transcription initiation factor TFIID 150 kDa subunit; Transcription initiation factor TFIID subunit 2
Gene Aliases: CIF150; MRT40; TAF2; TAF2B; TAFII150
UniProt ID: (Human) Q6P1X5
Entrez Gene ID: (Human) 6873
transcription factor activity, RNA polymerase II core promoter sequence-specific binding involved in preinitiation complex assembly
RNA polymerase II transcription factor activity, TBP-class protein binding, involved in preinitiation complex assembly
chromatin binding
protein binding
metallopeptidase activity
zinc ion binding
sequence-specific DNA binding
transcription regulatory region DNA binding
G2/M transition of mitotic cell cycle
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
proteolysis
response to organic cyclic compound
positive regulation of transcription from RNA polymerase II promoter
RNA polymerase II transcriptional preinitiation complex assembly
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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