Invitrogen

TAL-1 Monoclonal Antibody (2TL242), eBioscience™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

14-9101-82

Applications
Tested Dilution
Publications

Western Blot (WB)

5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL
-

Immunocytochemistry (ICC/IF)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2TL242

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572922

Product Specific Information

Description: The monoclonal antibody 2TL242 recognizes human TAL-1. The TAL-1 protein is encoded by the tal-1 (T-acute leukemia or stem cell leukemia (SCL)) gene and functions as a 42 kDa basic helix loop helix transcription factor involved in mediating cell growth and differentation. Rearrangements of the tal-1 gene are common in human T-cell acute leukemias (T-ALLs) and result in aberrant transcription in leukemic cells. TAL-1 expression in leukemic cells is localized to the nucleus although both nuclear and cytoplasmic expression has been shown in normal cells such as erythroid precursors, megakaryocytes, endothelial cells, and macrophages scattered throughout tissues such as the tonsil, cerebellum, kidney, lung and spleen. TAL-1 is known to regulate both embryonic and adult hematopoiesis and more recently was found to regulate proliferation and differentiation of common myeloid precursors to monocytes and ultimately macrophages within tissue.

Applications Reported: This 2TL242 antibody has been reported for use in western blotting, microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This 2TL242 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval at less than or equal to 20 µg/mL. This 2TL242 antibody has also been tested by western blot of reduced Jurkat cell lysate at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TAL-1 is a basic helix-loop-helix transcription. It regulates differentiation and survival during hemopoiesis. TAL-1 is also implicated in the genesis of hemopoietic malignancies and it may play an important role in hemopoietic differentiation. It serves as a positive regulator of eryhtroid differentiation. Mutations are associated with T-cell leukemia and melanoma. TAL-1 is know to bind to the LIM domain containing protein Rhombotin-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bHLHa17; Class A basic helix-loop-helix protein 17; HGNC:11556; Stem cell protein; T-cell acute lymphocytic leukemia 1; T-cell acute lymphocytic leukemia protein 1; T-cell leukemia/lymphoma protein 5; TAL-1; tal-1 product

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Gene Aliases: BHLHA17; SCL; tal-1; TAL1; TCL5

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UniProt ID: (Human) P17542

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Entrez Gene ID: (Human) 6886

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding enzyme binding histone deacetylase binding transcription regulatory region DNA binding protein heterodimerization activity E-box binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter angiogenesis transcription, DNA-templated regulation of transcription from RNA polymerase II promoter locomotory behavior spinal cord association neuron differentiation hemopoiesis erythrocyte differentiation megakaryocyte differentiation platelet formation basophil differentiation positive regulation of protein complex assembly embryonic hemopoiesis megakaryocyte development regulation of cell proliferation erythrocyte maturation cell fate commitment positive regulation of erythrocyte differentiation positive regulation of chromatin assembly or disassembly positive regulation of transcription, DNA-templated positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of cell division astrocyte fate commitment definitive hemopoiesis hemangioblast cell differentiation hematopoietic stem cell differentiation regulation of mast cell differentiation regulation of stem cell population maintenance
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