Invitrogen

TAMRA Monoclonal Antibody (5G5)

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TAMRA Antibody (MA1-041) in WB

Western blot analysis was performed by loading various amounts of purified TAMRA-BSA protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing TAMRA (Product # MA1-041) at a dilution of 1:1000 for 1hour at room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of ... View More

TAMRA Antibody in Immunoprecipitation (IP)

Product Image Gallery

TAMRA Antibody — Figure 8 Inhibition of translation using cycloheximide and rapamycin reduces the dendritic spine density. ( a ) Cycloheximide (Chx, 10 muM) and rapamycin (Rapa, 10 nM) treatment for 6 h inhibited protein synthesis of cultured neurons as revealed by the AHA signals with the TAMRA antibody. Images have been cropped for presentation. Full-size images are presented in Supplementary Fig. 9 . ( b ) Cultured neurons transfected with GFP-actin to outline dendritic spines were treated with Chx and Rapa for 6 or 24 h before immunostaining with the GFP antibody. Veh, vehicle. Scale bars: original, 20 mum; enlarged, 2 mum. The sample sizes ( n ) of the examined dendrites are indicated. The data from three independent experiments are presented as the mean+s.e.m. (error bars). * P <0.05; ** P <0.01; *** P <0.001; one-way ANOVA. {TM}

Product Details

MA1-041

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

10 µg

View 3 publications 3 publications

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5G5

Immunogen

TAMRA molecule

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536728

Product Specific Information

MA1-041 detects TAMRA in samples and has been used in western blotting and immunoprecipitation applications.

Target Information

The red-fluorescent tetramethylrhodamine (TAMRA) azide can be used as a reactive-azido group (Staudinger reagents) that facilitates multiplex labeling when used with phosphine- or alkyne-derivatized tags. In addition, TAMRA fluorophore has been used to label and detect active serine hydrolases in samples by fluorescent gel imaging, capillary electrophoresis or mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Axonal generation of amyloid-β from palmitoylated APP in mitochondria-associated endoplasmic reticulum membranes.

Cell reports

Bhattacharyya R,Black SE,Lotlikar MS,Fenn RH,Jorfi M,Kovacs DM,Tanzi RE

MA1-041 was used in Metabolic Labeling to suggest therapeutic strategies for reducing axonal release of Aβ and attenuating β-amyloid pathology in AD.

Tue May 18 00:00:00 UTC 2021

Activity-based protein profiling guided identification of urine proteinase 3 activity in subclinical rejection after renal transplantation.

Clinical proteomics

Navarrete M,Korkmaz B,Guarino C,Lesner A,Lao Y,Ho J,Nickerson P,Wilkins JA

MA1-041 was used in Sample Preparation to evaluate serine hydrolase enzyme activities to identify any underlying differences in activities during subclinical versus clinical rejection.

Fri Apr 15 00:00:00 UTC 2022

Activity-based protein profiling of rice (Oryza sativa L.) bran serine hydrolases.

Scientific reports

Dolui AK,Vijayakumar AK,Rajasekharan R,Vijayaraj P

Published figure using TAMRA monoclonal antibody (Product # MA1-041) in Immunoprecipitation

Wed Sep 16 00:00:00 UTC 2020

Vcp Overexpression and Leucine Supplementation Increase Protein Synthesis and Improve Fear Memory and Social Interaction of Nf1 Mutant Mice.

Cell reports

Shih YT,Huang TN,Hu HT,Yen TL,Hsueh YP

MA1-041 was used in Western Blotting to suggest that leucine can effectively enter the brain and increase protein synthesis and dendritic spine density of Nf1+/- neurons.

Tue Jun 30 00:00:00 UTC 2020

Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation.

The Journal of clinical investigation

Chennupati V,Veiga DF,Maslowski KM,Andina N,Tardivel A,Yu EC,Stilinovic M,Simillion C,Duchosal MA,Quadroni M,Roberts I,Sankaran VG,MacDonald HR,Fasel N,Angelillo-Scherrer A,Schneider P,Hoang T,Allam R

MA1-041 was used in Western Blotting to reveal an unsuspected role for ribonuclease inhibitor 1 in the control of GATA1 mRNA translation and erythropoiesis.

Mon Apr 02 00:00:00 UTC 2018

Methods for the Detection, Study, and Dynamic Profiling of O-GlcNAc Glycosylation.

Methods in enzymology

Thompson JW,Griffin ME,Hsieh-Wilson LC

MA1-041 was used in Immunoprecipitation to elucidate the key roles of O-linked Β-N-acetylglucosamine glycosylation in the regulation of individual proteins, complex cellular processes, and disease.

Mon Jan 28 00:00:00 UTC 2019

Nerve-targeted probes for fluorescence-guided intraoperative imaging.

Theranostics

Hingorani DV,Whitney MA,Friedman B,Kwon JK,Crisp JL,Xiong Q,Gross L,Kane CJ,Tsien RY,Nguyen QT

MA1-041 was used in Immunocytochemistry-immunoflourescence to investigate whether fluorescent labelling of nerves can enhance the contrast between nerves and adjacent tissue during surgery.

Wed Sep 04 00:00:00 UTC 2019

Nerve-targeted probes for fluorescence-guided intraoperative imaging.

Theranostics

Hingorani DV,Whitney MA,Friedman B,Kwon JK,Crisp JL,Xiong Q,Gross L,Kane CJ,Tsien RY,Nguyen QT

MA1-041 was used in Immunocytochemistry-immunoflourescence to investigate whether fluorescent labelling of nerves can enhance the contrast between nerves and adjacent tissue during surgery.

Wed Sep 04 00:00:00 UTC 2019

VCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation.

Nature communications

Shih YT,Hsueh YP

MA1-041 was used in western blot to show how ATL1 and VCP work together to regulate endoplasmic reticulum and protein synthesis for dendritic spine formation

Thu Mar 17 00:00:00 UTC 2016

High-resolution functional proteomics by active-site peptide profiling.

Proceedings of the National Academy of Sciences of the United States of America

Okerberg ES,Wu J,Zhang B,Samii B,Blackford K,Winn DT,Shreder KR,Burbaum JJ,Patricelli MP

MA1-041 was used in immunoprecipitation to enrich for TAMRA-labeled peptides and then eluted and analyzed LC-MS/MS.

Tue Apr 05 00:00:00 UTC 2005

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