Proteintech

TBP Monoclonal Antibody (2H3B2)

TBP Antibody in Immunocytochemistry (ICC/IF)

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TBP Antibody (66166-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBP antibody (Product # 66166-1-Ig) (Clone: 2H3B2) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

TBP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TBP Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

66166-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:16,000

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:325-1:1300

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2H3B2

Immunogen

TBP Fusion Protein Ag12383 (1-338 aa encoded by BC110341)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).

Immunogen sequence: MDQNNSLPP YAQGLASPQG AMTPGIPIFS PMMPYGTGLT PQPIQNTNSL SILEEQQRQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQAVAAA AVQQSTSQQA TQGTSGQAPQ LFHSQTLTTA PLPGTTPLYP SPMTPMTPIT PATPASESSG IVPQLQNIVS TVNLGCKLDL KTIALRARNA EYNPKRFAAV IMRIREPRTT ALIFSSGKMV CTGAKSEEQS RLAARKYARV VQKLGFPAKF LDFKIQNMVG SCDVKFPIRL EGLVLTHQQF SSYEPELFPG LIYRMIKPRI VLLIFVSGKV VLTGAKVRAE IYEAFENIYP ILKGFRKTT (1-338 aa encoded by BC110341 )

Target Information

RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins, such as RHOA, RAC1, and CDC42.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TLR2-mediated NF-κB signaling pathway is involved in PPV1-induced apoptosis in PK-15 cells.

Veterinary research communications

Xu M,Jin X,Zhang C,Liao H,Wang P,Zhou Y,Song Y,Xia L,Wang L

Thu Jun 01 00:00:00 UTC 2023

3D-printed hydrogel particles containing PRP laden with TDSCs promote tendon repair in a rat model of tendinopathy.

Journal of nanobiotechnology

Li C,Wang J,Yang W,Yu K,Hong J,Ji X,Yao M,Li S,Lu J,Chen Y,Yan S,Wu H,Ma C,Yu X,Jiang G,Liu A

Sat Jun 03 00:00:00 UTC 2023

3D-printed hydrogel particles containing PRP laden with TDSCs promote tendon repair in a rat model of tendinopathy.

Journal of nanobiotechnology

Li C,Wang J,Yang W,Yu K,Hong J,Ji X,Yao M,Li S,Lu J,Chen Y,Yan S,Wu H,Ma C,Yu X,Jiang G,Liu A

Sat Jun 03 00:00:00 UTC 2023

YY1 (Yin-Yang 1), a transcription factor regulating systemic inflammation, is involved in cognitive impairment of depression.

Psychiatry and clinical neurosciences

Lu J,Jin K,Jiao J,Liu R,Mou T,Chen B,Zhang Z,Jiang C,Zhao H,Wang Z,Zhou R,Huang M

Wed Mar 01 00:00:00 UTC 2023

Caspase-1-Dependent Pyroptosis Mediates Adjuvant Activity of Platycodin D as an Adjuvant for Intramuscular Vaccines.

Cells

Zhu L,Han Z,He Y,Sun H

Sat Jan 01 00:00:00 UTC 2022

G-quadruplexes sense natural porphyrin metabolites for regulation of gene transcription and chromatin landscapes.

Genome biology

Li C,Yin Z,Xiao R,Huang B,Cui Y,Wang H,Xiang Y,Wang L,Lei L,Ye J,Li T,Zhong Y,Guo F,Xia Y,Fang P,Liang K

Thu Dec 15 00:00:00 UTC 2022

PPARα regulates the expression of human arylacetamide deacetylase involved in drug hydrolysis and lipid metabolism.

Biochemical pharmacology

Morikawa T,Fukami T,Gotoh-Saito S,Nakano M,Nakajima M

Sun May 01 00:00:00 UTC 2022

RPAP2 regulates a transcription initiation checkpoint by inhibiting assembly of pre-initiation complex.

Cell reports

Wang X,Qi Y,Wang Z,Wang L,Song A,Tao B,Li J,Zhao D,Zhang H,Jin Q,Jiang YZ,Chen FX,Xu Y,Chen X

Tue Apr 26 00:00:00 UTC 2022

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Virulence

Jie W,Rui-Fen Z,Zhong-Xiang H,Yan W,Wei-Na L,Yong-Ping M,Jing S,Jing-Yi C,Wan-Hong L,Xiao-Hua H,Zhi L,Yan S

Thu Dec 01 00:00:00 UTC 2022

TFEB Promotes Prostate Cancer Progression via Regulating ABCA2-Dependent Lysosomal Biogenesis.

Frontiers in oncology

Zhu X,Zhuo Y,Wu S,Chen Y,Ye J,Deng Y,Feng Y,Liu R,Cai S,Zou Z,Wang B,Wu CL,Zeng G,Zhong W

Thu Oct 21 00:00:00 UTC 2021

Ligand-induced native G-quadruplex stabilization impairs transcription initiation.

Genome research

Li C,Wang H,Yin Z,Fang P,Xiao R,Xiang Y,Wang W,Li Q,Huang B,Huang J,Liang K

Wed Sep 01 00:00:00 UTC 2021

Adenosine deaminases acting on RNA modulate the expression of the human pregnane X receptor.

Drug metabolism and pharmacokinetics

Takemoto S,Nakano M,Nozaki K,Fukami T,Nakajima M

Thu Apr 01 00:00:00 UTC 2021

Shift in MSL1 alternative polyadenylation in response to DNA damage protects cancer cells from chemotherapeutic agent-induced apoptosis.

Cell reports

Kunisky AK,Anyaeche VI,Herron RS,Park CY,Hwang HW

Tue Oct 12 00:00:00 UTC 2021

Downregulation of the histone methyltransferase SETD2 promotes imatinib resistance in chronic myeloid leukaemia cells.

Cell proliferation

Sheng Y,Ji Z,Zhao H,Wang J,Cheng C,Xu W,Wang X,He Y,Liu K,Li L,Voeltzel T,Maguer-Satta V,Gao WQ,Zhu HH

Mon Jul 01 00:00:00 UTC 2019

Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and β-Catenin-Independent Targets by Proteomics.

Molecular cancer research : MCR

Popow O,Paulo JA,Tatham MH,Volk MS,Rojas-Fernandez A,Loyer N,Newton IP,Januschke J,Haigis KM,Näthke I

Sun Sep 01 00:00:00 UTC 2019

Matrine inhibits IL-1β secretion in primary porcine alveolar macrophages through the MyD88/NF-κB pathway and NLRP3 inflammasome.

Veterinary research

Sun P,Sun N,Yin W,Sun Y,Fan K,Guo J,Khan A,He Y,Li H

Fri Jul 12 00:00:00 UTC 2019

Loss of adenomatous polyposis coli function renders intestinal epithelial cells resistant to the cytokine IL-22.

PLoS biology

Chen Y,Vandereyken M,Newton IP,Moraga I,Näthke IS,Swamy M

Fri Nov 01 00:00:00 UTC 2019

Adaptive EGF expression sensitizes pancreatic cancer cells to ionizing radiation through activation of the cyclin D1/P53/PARP pathway.

International journal of oncology

Liu X,Chen H,Hou Y,Ma X,Ye M,Huang R,Hu B,Cao H,Xu L,Liu M,Li L,Gao J,Bai Y

Mon Apr 01 00:00:00 UTC 2019

Imbalance of a KLF4-miR-7 auto-regulatory feedback loop promotes prostate cancer cell growth by impairing microRNA processing.

American journal of cancer research

Wei LZ,Wang YQ,Chang YL,An N,Wang X,Zhou PJ,Zhu HH,Fang YX,Gao WQ

Thu Oct 01 00:00:00 UTC 2020

Imbalance of a KLF4-miR-7 auto-regulatory feedback loop promotes prostate cancer cell growth by impairing microRNA processing.

American journal of cancer research

Wei LZ,Wang YQ,Chang YL,An N,Wang X,Zhou PJ,Zhu HH,Fang YX,Gao WQ

Thu Oct 01 00:00:00 UTC 2020

B Subunit of Human Chorionic Gonadotropin Promotes Tumor Invasion and Predicts Poor Prognosis of Early-Stage Colorectal Cancer.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Li J,Yin M,Song W,Cui F,Wang W,Wang S,Zhu H

Fri Mar 16 00:00:00 UTC 2018

B Subunit of Human Chorionic Gonadotropin Promotes Tumor Invasion and Predicts Poor Prognosis of Early-Stage Colorectal Cancer.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Li J,Yin M,Song W,Cui F,Wang W,Wang S,Zhu H

Fri Mar 16 00:00:00 UTC 2018

Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.

Molecular and cellular endocrinology

Li D,Wang X,Lan X,Li Y,Liu L,Yi J,Li J,Sun Q,Wang Y,Li H,Zhong N,Holmdahl R,Lu S

Sun Feb 15 00:00:00 UTC 2015

Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.

Molecular and cellular endocrinology

Li D,Wang X,Lan X,Li Y,Liu L,Yi J,Li J,Sun Q,Wang Y,Li H,Zhong N,Holmdahl R,Lu S

Sun Feb 15 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: MGC117320; MGC126054; MGC126055; RP1-191N21.3; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; tata-box-binding; TATA-box-binding protein; TFIID core protein; Transcription initiation factor TFIID TBP subunit

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Gene Aliases: GTF2D; GTF2D1; HDL4; SCA17; TBP; TF2D; TFIID

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UniProt ID: (Human) P20226, (Mouse) P29037

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Entrez Gene ID: (Human) 6908, (Mouse) 21374, (Rat) 117526

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II repressing transcription factor binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding enzyme binding transcription regulatory region DNA binding repressing transcription factor binding DNA binding

Process(es)

transcription, DNA-templated DNA-templated transcription, initiation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter transcription from RNA polymerase III promoter positive regulation of transcription, DNA-templated transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase I promoter termination of RNA polymerase I transcription transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter spermatogenesis viral process snRNA transcription from RNA polymerase II promoter positive regulation of gene expression, epigenetic regulation of signal transduction by p53 class mediator

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TBP Monoclonal Antibody (2H3B2)

TBP Antibody (66166-1-IG) in ICC/IF

Product Details

66166-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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