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Invitrogen
TBX5 Polyclonal Antibody
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FIGURE: 1 / 5
TBX5 Antibody (42-6500) in ICC/IF
Immunofluorescence analysis of TBX5 Polyclonal Antibody was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TBX5 Rabbit Polyclonal Antibody (Product # 42-6500) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conju... View More
Product Details
42-6500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from an internal region of the human, predicted dog, and predicted chimpanzee Tbx5 proteins, which differ from mouse and rat by one conservative amino acid change
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533533
Target Information
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: T-box protein 5; T-box transcription factor TBX5
Gene Aliases: HOS; TBX5
UniProt ID: (Human) Q99593, (Mouse) P70326
Entrez Gene ID: (Human) 6910, (Mouse) 21388
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
sequence-specific DNA binding
morphogenesis of an epithelium
bundle of His development
atrioventricular valve morphogenesis
endocardial cushion development
cardiac left ventricle formation
ventricular septum development
transcription initiation from RNA polymerase II promoter
cell-cell signaling
pattern specification process
heart development
negative regulation of cell proliferation
negative regulation of epithelial to mesenchymal transition
lung development
embryonic limb morphogenesis
negative regulation of cell migration
embryonic forelimb morphogenesis
forelimb morphogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of cardioblast differentiation
cardiac muscle cell differentiation
pericardium development
negative regulation of cardiac muscle cell proliferation
positive regulation of cardiac muscle cell proliferation
transdifferentiation
atrial septum morphogenesis
cell migration involved in coronary vasculogenesis
positive regulation of secondary heart field cardioblast proliferation
regulation of execution phase of apoptosis
ventricular cardiac muscle tissue development
atrial septum development
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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