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FIGURE: 1 / 1
TCF12 Antibody (TA809695) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TCF12 (RC220164, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TCF12. Positive lysates LY403720 (100 µg) and LC403720 (20 µg) can be purchased separately from OriGene.
Product Details
TA809695
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1G10
Immunogen
Full length human recombinant protein of human TCF12 produced in E.coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb20; Class B basic helix-loop-helix protein 20; DNA-binding protein HTF4; E-box-binding protein; helix-loop-helix transcription factor 4; TCF-12; Transcription factor 12; Transcription factor HTF-4
Gene Aliases: BHLHB20; CRS3; HEB; HsT17266; HTF4; TCF-12; TCF12
UniProt ID: (Human) Q99081
Entrez Gene ID: (Human) 6938
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
enhancer binding
cAMP response element binding
protein homodimerization activity
bHLH transcription factor binding
transcription regulatory region DNA binding
SMAD binding
protein heterodimerization activity
E-box binding
HMG box domain binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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