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Product Details
PA5-85281
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human TCF3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792424
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (85%), Rat (82%).
Positive Control: Jurkat, NCI-H929, BCL-1.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TCF3 (E2A) is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb21; Class B basic helix-loop-helix protein 21; helix-loop-helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; MGC129647; MGC129648; negative vitamin D response element-binding protein; NOL1-TCF3 fusion; TCF-3; Transcription factor 3; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); transcription factor 3 variant 3; Transcription factor A1; Transcription factor E2-alpha; transcription factor E2a; Transcription factor ITF-1; VDR interacting repressor; vitamin D receptor-interacting repressor
Gene Aliases: A1; AA408400; AGM8; ALF2; AW209082; BHLHB21; E12; E12/E47; E2A; E47; ITF1; ME2; Pan1; Pan2; TCF-3; TCF3; Tcfe2a; VDIR
UniProt ID: (Human) P15923, (Mouse) P15806
Entrez Gene ID: (Human) 6929, (Mouse) 21423
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
transcription factor binding
mitogen-activated protein kinase kinase kinase binding
enhancer binding
protein homodimerization activity
bHLH transcription factor binding
protein heterodimerization activity
repressing transcription factor binding
vitamin D response element binding
E-box binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
chromatin binding
PDZ domain binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein dimerization activity
negative regulation of transcription from RNA polymerase II promoter
natural killer cell differentiation
B cell lineage commitment
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
gastrulation
nervous system development
positive regulation of gene expression
lymphocyte differentiation
cell differentiation
positive regulation of B cell proliferation
response to lipopolysaccharide
T cell differentiation in thymus
immunoglobulin V(D)J recombination
response to drug
histone H3 acetylation
histone H4 acetylation
positive regulation of neuron differentiation
positive regulation of cell cycle
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell development
Peyer's patch development
protein stabilization
positive regulation of sequence-specific DNA binding transcription factor activity
regulation of G1/S transition of mitotic cell cycle
B cell differentiation
positive regulation of muscle cell differentiation
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