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TCF3 Antibody (MA5-32092) in Flow
Flow Cytometric analysis of TCF3 in 293 cells using a TCF3 Monoclonal Antibody (Product # MA5-32092) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Product Details
MA5-32092
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SY0258
Immunogen
Synthetic peptide within Human TCF3 aa 71-120
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809385
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
TCF3 (E2A) is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb21; Class B basic helix-loop-helix protein 21; helix-loop-helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; MGC129647; MGC129648; negative vitamin D response element-binding protein; NOL1-TCF3 fusion; TCF-3; Transcription factor 3; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); transcription factor 3 variant 3; Transcription factor E2-alpha; Transcription factor ITF-1; VDR interacting repressor; vitamin D receptor-interacting repressor
Gene Aliases: AGM8; BHLHB21; E2A; E47; ITF1; TCF-3; TCF3; VDIR
UniProt ID: (Human) P15923
Entrez Gene ID: (Human) 6929
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
transcription factor binding
mitogen-activated protein kinase kinase kinase binding
enhancer binding
protein homodimerization activity
bHLH transcription factor binding
protein heterodimerization activity
repressing transcription factor binding
vitamin D response element binding
E-box binding
negative regulation of transcription from RNA polymerase II promoter
B cell lineage commitment
transcription, DNA-templated
regulation of transcription, DNA-templated
B cell differentiation
immunoglobulin V(D)J recombination
positive regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of muscle cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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