Invitrogen

TCF3/TCF4 Monoclonal Antibody (6F12-3)

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-25418

Applications
Tested Dilution
Publications

Western Blot (WB)

1-4 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6F12-3

Immunogen

His-tagged fusion protein corresponding to amino acids 31-331 of human TCF.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.53 mg/mL

Purification

Protein G

Storage buffer

TBS, pH 7.4, with 30% glycerol

Contains

0.08% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_795485

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TCF 4 (T cell factor 4) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with b catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 4 generates transcriptionally active complexes with b catenin in colon carcinoma cells. Expression of TCF 4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF 4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bHLHb19; bHLHb21; Class A helix-loop-helix transcription factor ME2; Class B basic helix-loop-helix protein 19; Class B basic helix-loop-helix protein 21; helix-loop-helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Immunoglobulin transcription factor 2; immunoglobulin transcription factor-2; ITF-1; ITF-2; Kappa-E2-binding factor; MITF-2; negative vitamin D response element-binding protein; NOL1-TCF3 fusion; SEF-2; SL3-3 enhancer factor 2; TCF-3; TCF-4; Transcription factor 3; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); transcription factor 3 variant 3; Transcription factor 4; Transcription factor 4 (Immunoglobulin transcription factor 2) (ITF-2) (MITF-2) (SL3-3 enhancer factor 2) (SEF-2) (Class A helix-loop-helix transcription factor ME2); transcription factor 4, isoform C; transcription factor 4, isoform D; transcription factor 4, isoform E; transcription factor 4, isoform L; transcription factor 4, isoform M; transcription factor 4, isoform R; Transcription factor A1; Transcription factor E2-alpha; transcription factor E2a; Transcription factor ITF-1; VDR interacting repressor; vitamin D receptor-interacting repressor

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Gene Aliases: 5730422P05Rik; A1; AA408400; AGM8; ALF2; ASP-I2; AW209082; BHLHB19; BHLHB21; E12; E12/E47; E2-2; E2.2; E2A; E47; FECD3; ITF-2; ITF-2b; ITF1; ITF2; ME2; MITF-2A; MITF-2B; Pan1; Pan2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-3; TCF-4; TCF3; TCF4; Tcfe2a; TFE; VDIR

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UniProt ID: (Human) P15923, (Human) P15884, (Mouse) P15806, (Mouse) Q60722

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Entrez Gene ID: (Human) 6929, (Human) 6925, (Mouse) 21423, (Mouse) 21413

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding mitogen-activated protein kinase kinase kinase binding enhancer binding protein homodimerization activity bHLH transcription factor binding protein heterodimerization activity repressing transcription factor binding vitamin D response element binding E-box binding transcription factor activity, sequence-specific DNA binding, RNA polymerase recruiting transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, TFIIB-class binding TFIIB-class transcription factor binding protein C-terminus binding identical protein binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding chromatin binding PDZ domain binding sequence-specific DNA binding transcription regulatory region DNA binding protein dimerization activity double-stranded DNA binding transcription corepressor activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter natural killer cell differentiation B cell lineage commitment transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter gastrulation nervous system development positive regulation of gene expression lymphocyte differentiation cell differentiation positive regulation of B cell proliferation response to lipopolysaccharide T cell differentiation in thymus immunoglobulin V(D)J recombination response to drug histone H3 acetylation histone H4 acetylation positive regulation of neuron differentiation positive regulation of cell cycle positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell development Peyer's patch development protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity regulation of G1/S transition of mitotic cell cycle DNA-templated transcription, initiation transcription initiation from RNA polymerase II promoter protein-DNA complex assembly B cell differentiation positive regulation of muscle cell differentiation multicellular organism development
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