Invitrogen

TCF4/TCF12 Polyclonal Antibody

View all (2) TCF4/TCF12 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-36151

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 580-630 of human TCF-4
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2553395

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 60 and 72 kDa.

Purity is >95% by SDS-PAGE.

Target Information

T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group(HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to beta-catenin in a cooperative manner. This TCF-4/ beta-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bHLHb19; bHLHb20; Class A helix-loop-helix transcription factor ME1; Class A helix-loop-helix transcription factor ME2; Class B basic helix-loop-helix protein 19; Class B basic helix-loop-helix protein 20; DNA-binding protein HTF4; E-box-binding protein; helix-loop-helix transcription factor 4; Immunoglobulin transcription factor 2; immunoglobulin transcription factor-2; ITF-2; MITF-2; R8f DNA-binding protein; RITF-2; Salivary-specific cAMP response element-binding protein alpha; SCBP alpha; SCBP-alpha; SEF-2; SL3-3 enhancer factor 2; TCF-12; TCF-4; Transcription factor 12; Transcription factor 4; Transcription factor 4 (Immunoglobulin transcription factor 2) (ITF-2) (MITF-2) (SL3-3 enhancer factor 2) (SEF-2) (Class A helix-loop-helix transcription factor ME2); transcription factor 4, isoform C; transcription factor 4, isoform D; transcription factor 4, isoform E; transcription factor 4, isoform L; transcription factor 4, isoform M; transcription factor 4, isoform R; Transcription factor HTF-4

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Gene Aliases: 5730422P05Rik; A130037E08Rik; ALF1; ASP-I2; BHLHB19; BHLHB20; CRS3; E2-2; E2.2; FECD3; HEB; HEBAlt; HsT17266; HTF-4; HTF4; ITF-2; ITF-2b; ITF2; ME1; ME2; Meb; MITF-2A; MITF-2B; PTHS; REB; SCBPA; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-12; TCF-4; TCF12; TCF4; TFE

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UniProt ID: (Human) Q99081, (Human) P15884, (Mouse) Q61286, (Rat) P51514, (Mouse) Q60722, (Rat) Q62655

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Entrez Gene ID: (Human) 6938, (Human) 6925, (Mouse) 21406, (Rat) 25720, (Mouse) 21413, (Rat) 84382

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding enhancer binding cAMP response element binding protein homodimerization activity bHLH transcription factor binding transcription regulatory region DNA binding SMAD binding protein heterodimerization activity E-box binding HMG box domain binding transcription factor activity, sequence-specific DNA binding, RNA polymerase recruiting transcription factor activity, TFIIB-class binding TFIIB-class transcription factor binding DNA binding protein C-terminus binding identical protein binding protein dimerization activity chromatin binding double-stranded DNA binding transcription corepressor activity
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development nervous system development positive regulation of gene expression cell differentiation positive regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter DNA-templated transcription, initiation transcription initiation from RNA polymerase II promoter protein-DNA complex assembly negative regulation of transcription from RNA polymerase II promoter regulation of transcription from RNA polymerase II promoter immune response muscle organ development
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