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Invitrogen
TCF7 Recombinant Rabbit Monoclonal Antibody (18H15L9)
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FIGURE: 1 / 5
TCF7 Antibody (702957) in ICC/IF
Knockdown of TCF7 was achieved by transfecting HeLa cells with specific siRNA (Silencer® select Product # s13877, s13878). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a-d), transfected with TCF7 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-TCF Recombinant Rabbit Monoclonal Antibody (Product # 702957, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More
Product Details
702957
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
18H15L9
Immunogen
Protein corresponding to human TCF7 [aa1-aa384]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2784552
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
TCF7 (transcriptional factor 7) is a transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ and CD8+ immature thymocytes. Isoforms for TCF7 lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. TCF7 binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. It may also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HMG-box; OTTHUMP00000159390; OTTHUMP00000159391; T-cell factor 1; T-cell specific; T-cell-specific transcription factor 1; TCF-7; Transcription factor 7
Gene Aliases: TCF-1; TCF1; TCF7
UniProt ID: (Human) P36402
Entrez Gene ID: (Human) 6932
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
immune response
neural tube development
embryonic genitalia morphogenesis
T cell receptor V(D)J recombination
regulation of cell proliferation
canonical Wnt signaling pathway involved in negative regulation of apoptotic process
alpha-beta T cell differentiation
embryonic digestive tract morphogenesis
embryonic hindgut morphogenesis
canonical Wnt signaling pathway
cellular response to interleukin-4
beta-catenin-TCF complex assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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