Invitrogen

TCF7 Monoclonal Antibody (C.725.7)

TCF7 Antibody in Immunocytochemistry (ICC/IF)

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TCF7 Antibody (MA5-14965) in ICC/IF

Immunofluorescent analysis of TCF1 in DLD-1 cells using a TCF1 monoclonal antibody (Product # MA5-14965) (green). Actin filaments are labeled with a fluorescent red phalloidin.

TCF7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TCF7 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

TCF7 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of TCF7 was observed in MOLT-4, Jurkat, HeLa, SW480 in comparison to U2OS and LNCap and in Mouse Thymus as compared to Mouse Skeletal Muscle using Anti-TCF7 Monoclonal Antibody (C.725.7) (Product # MA5-14965) in Western Blot. {RE}

Product Details

MA5-14965

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:200-1:800

Flow Cytometry (Flow)

1:50-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

C.725.7

Immunogen

Synthetic peptide corresponding to a region surrounding Pro96 of human TCF1 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10983684

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with LEF1.

Target Information

TCF7 (transcriptional factor 7) is a transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ and CD8+ immature thymocytes. Isoforms for TCF7 lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. TCF7 binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. It may also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by TCF7 and CTNNB1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

THBS1-producing tumor-infiltrating monocyte-like cells contribute to immunosuppression and metastasis in colorectal cancer.

Nature communications

Omatsu M,Nakanishi Y,Iwane K,Aoyama N,Duran A,Muta Y,Martinez-Ordoñez A,Han Q,Agatsuma N,Mizukoshi K,Kawai M,Yamakawa G,Namikawa M,Hamada K,Fukunaga Y,Utsumi T,Sono M,Masuda T,Hata A,Araki O,Nagao M,Yoshikawa T,Ogawa S,Hiramatsu Y,Tsuda M,Maruno T,Kogame T,Kasashima H,Kakiuchi N,Nakagawa MM,Kawada K,Yashiro M,Maeda K,Saito Y,Matozaki T,Fukuda A,Kabashima K,Obama K,Ogawa S,Sheibani N,Diaz-Meco MT,Moscat J,Seno H

MA5-14965 was used in Immunohistochemistry to establish THBS1 as a potential biomarker for identifying mesenchymal CRC and as a critical suppressor of antitumor immunity that contributes to the progression of this malignancy with a poor prognosis.

Mon Sep 25 00:00:00 UTC 2023

Clonally expanded CD8 T cells characterize amyotrophic lateral sclerosis-4.

Nature

Campisi L,Chizari S,Ho JSY,Gromova A,Arnold FJ,Mosca L,Mei X,Fstkchyan Y,Torre D,Beharry C,Garcia-Forn M,Jiménez-Alcázar M,Korobeynikov VA,Prazich J,Fayad ZA,Seldin MM,De Rubeis S,Bennett CL,Ostrow LW,Lunetta C,Squatrito M,Byun M,Shneider NA,Jiang N,La Spada AR,Marazzi I

MA5-14965 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to unravel disease mechanisms and as a potential biomarker of disease state.

Wed Jun 01 00:00:00 UTC 2022

Oncogenic cooperation between TCF7-SPI1 and NRAS(G12D) requires β-catenin activity to drive T-cell acute lymphoblastic leukemia.

Nature communications

Van Thillo Q,De Bie J,Seneviratne JA,Demeyer S,Omari S,Balachandran A,Zhai V,Tam WL,Sweron B,Geerdens E,Gielen O,Provost S,Segers H,Boeckx N,Marshall GM,Cheung BB,Isobe K,Kato I,Takita J,Amos TG,Deveson IW,McCalmont H,Lock RB,Oxley EP,Garwood MM,Dickins RA,Uyttebroeck A,Carter DR,Cools J,de Bock CE

MA5-14965 was used in Western Blot to show that targeting the TCF7-SPI1 fusion in vivo with a doxycycline-inducible knockdown results in increased differentiation.

Tue Jul 06 00:00:00 UTC 2021

Oncogenic cooperation between TCF7-SPI1 and NRAS(G12D) requires β-catenin activity to drive T-cell acute lymphoblastic leukemia.

Nature communications

MA5-14965 was used in Western Blot to show that targeting the TCF7-SPI1 fusion in vivo with a doxycycline-inducible knockdown results in increased differentiation.

Tue Jul 06 00:00:00 UTC 2021

Chronic expression of p16^INK4a in the epidermis induces Wnt-mediated hyperplasia and promotes tumor initiation.

Nature communications

Azazmeh N,Assouline B,Winter E,Ruppo S,Nevo Y,Maly A,Meir K,Witkiewicz AK,Cohen J,Rizou SV,Pikarsky E,Luxenburg C,Gorgoulis VG,Ben-Porath I

MA5-14965 was used in Immunohistochemistry to investigate the effect of prolonged expression of transgenic p16INK4a in the mouse epidermis and the pathways involved in this effect.

Mon Jun 01 00:00:00 UTC 2020

Chronic expression of p16^INK4a in the epidermis induces Wnt-mediated hyperplasia and promotes tumor initiation.

Nature communications

Azazmeh N,Assouline B,Winter E,Ruppo S,Nevo Y,Maly A,Meir K,Witkiewicz AK,Cohen J,Rizou SV,Pikarsky E,Luxenburg C,Gorgoulis VG,Ben-Porath I

MA5-14965 was used in Immunohistochemistry to investigate the effect of prolonged expression of transgenic p16INK4a in the mouse epidermis and the pathways involved in this effect.

Mon Jun 01 00:00:00 UTC 2020

Intrinsic 4-1BB signals are indispensable for the establishment of an influenza-specific tissue-resident memory CD8 T-cell population in the lung.

Mucosal immunology

Zhou AC,Wagar LE,Wortzman ME,Watts TH

MA5-14965 was used in Flow cytometry/Cell sorting to show that intranasal administration of 4-1BBL along with influenza nucleoprotein in a replicationdefective adenovirus vector to influenza pre-immune mice induces a remarkably stable circulating effector memory CD8 T-cell population.

Fri Sep 01 00:00:00 UTC 2017

β-catenin is required for taste bud cell renewal and behavioral taste perception in adult mice.

PLoS genetics

Gaillard D,Bowles SG,Salcedo E,Xu M,Millar SE,Barlow LA

MA5-14965 was used in Immunohistochemistry to show that conditional β-catenin deletion in mouse taste progenitors leads to rapid depletion of progenitors and Shh+ precursors, which in turn causes taste bud loss, followed by loss of gustatory nerve fibers.

Tue Aug 01 00:00:00 UTC 2017

β-catenin is required for taste bud cell renewal and behavioral taste perception in adult mice.

PLoS genetics

Gaillard D,Bowles SG,Salcedo E,Xu M,Millar SE,Barlow LA

Tue Aug 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: HMG-box; OTTHUMP00000159390; OTTHUMP00000159391; T cell factor-1; T-cell factor 1; T-cell specific; T-cell-specific transcription factor 1; TCF-7; Transcription factor 7; transcription factor 7, T-cell specific

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Gene Aliases: AI465550; TCF-1; TCF1; TCF7

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UniProt ID: (Human) P36402, (Mouse) Q00417

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Entrez Gene ID: (Human) 6932, (Mouse) 21414

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Function(s)

core promoter binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding protein binding beta-catenin binding sequence-specific DNA binding transcription regulatory region DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription from RNA polymerase II promoter immune response neural tube development embryonic genitalia morphogenesis T cell receptor V(D)J recombination regulation of cell proliferation canonical Wnt signaling pathway involved in negative regulation of apoptotic process alpha-beta T cell differentiation embryonic digestive tract morphogenesis embryonic hindgut morphogenesis canonical Wnt signaling pathway cellular response to interleukin-4 beta-catenin-TCF complex assembly regulation of transcription, DNA-templated transcription from RNA polymerase II promoter Wnt signaling pathway generation of neurons

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TCF7 Monoclonal Antibody (C.725.7)

TCF7 Antibody (MA5-14965) in ICC/IF

Product Details

MA5-14965

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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