Invitrogen

TCR V gamma 9 Monoclonal Antibody (7A5), FITC

TCR V gamma 9 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

TCR V gamma 9 Antibody (TCR2720) in Flow

Flow cytometry analysis of TCR V gamma 9 on IPP and IL2 stimmunlated human PBMCs. Equal numbers of cells were stained with a FITC labeled TCR V gamma 9 monoclonal antibody (7A5) (Product # TCR2720) or FITC labeled control. 5 µL of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature. A representative 10,000 cells were acquired for each sample.

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Product Details

TCR2720

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL/test

View 8 publications 8 publications

ELISA (ELISA)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7A5

Immunogen

Human TCR Vgamma9

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS with 0.5% BSA

Contains

0.1% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_417094

Product Specific Information

TCR2720 targets TCR V gamma 9 in FACS applications and shows reactivity with Human, and Non-human primate samples.

The TCR2720 immunogen is human TCR Vgamma9.

Target Information

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Phenotypic and functional characterization of pharmacologically expanded Vγ9Vδ2 T cells in pigtail macaques.

iScience

Barber-Axthelm IM,Wragg KM,Esterbauer R,Amarasena TH,Barber-Axthelm VRB,Wheatley AK,Gibbon AM,Kent SJ,Juno JA

TCR2720 was used in Flow cytometry/Cell sorting to show the tissue distribution and phenotype of in vivo pharmacologically expanded Vγ9Vδ2 T cells can be altered based on the antigen administration route, with implications for tissue trafficking and the clinical efficacy of Vγ9Vδ2 T cell immunotherapeutics.

Fri Mar 17 00:00:00 UTC 2023

Defining TCRγδ lymphoproliferative disorders by combined immunophenotypic and molecular evaluation.

Nature communications

Teramo A,Binatti A,Ciabatti E,Schiavoni G,Tarrini G,Barilà G,Calabretto G,Vicenzetto C,Gasparini VR,Facco M,Petrini I,Grossi R,Pisanti N,Bortoluzzi S,Falini B,Tiacci E,Galimberti S,Semenzato G,Zambello R

TCR2720 was used in Flow cytometry/Cell sorting to show that an association between Vδ2/Vγ9 clonality and indolent course, while Vδ2/Vγ9 negativity correlates with symptomatic disease.

Wed Jun 08 00:00:00 UTC 2022

HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of Vγ2Vδ2 T cells.

Journal of leukocyte biology

Frencher JT,Shen H,Yan L,Wilson JO,Freitag NE,Rizzo AN,Chen CY,Chen ZW

TCR2720 was used in flow cytometry to examine the effect of HMBPP-deficient Listeria mutant immunization

Mon Dec 01 00:00:00 UTC 2014

Mechanisms of the antitumor activity of human Vγ9Vδ2 T cells in combination with zoledronic acid in a preclinical model of neuroblastoma.

Molecular therapy : the journal of the American Society of Gene Therapy

Di Carlo E,Bocca P,Emionite L,Cilli M,Cipollone G,Morandi F,Raffaghello L,Pistoia V,Prigione I

TCR2720 was used in blocking or activating experiment to study the mechanisms underlying the therapautic activity of T-cell receptor Vgamma9Vdelta2 lymphocytes and zoledronic acid in a neuroblastoma model

Wed May 01 00:00:00 UTC 2013

Phosphoantigen/IL2 expansion and differentiation of Vγ2Vδ2 T cells increase resistance to tuberculosis in nonhuman primates.

PLoS pathogens

Chen CY,Yao S,Huang D,Wei H,Sicard H,Zeng G,Jomaa H,Larsen MH,Jacobs WR,Wang R,Letvin N,Shen Y,Qiu L,Shen L,Chen ZW

TCR2720 was used in immunohistochemistry to study the increased Tuberculosis resistance of non-human primates following phoshoantigen/IL-2 mediated expansion and differentiation of VgammaVdelta2 T-cells

Thu Aug 01 00:00:00 UTC 2013

Multieffector-functional immune responses of HMBPP-specific Vγ2Vδ2 T cells in nonhuman primates inoculated with Listeria monocytogenes ΔactA prfA*.

Journal of immunology (Baltimore, Md. : 1950)

Ryan-Payseur B,Frencher J,Shen L,Chen CY,Huang D,Chen ZW

TCR2720 was used in flow cytometry to study multi-effector functional Vgamma2Vdelta2 T cell responses against Listeria monocytogenes in nonhuman primates

Wed Aug 01 00:00:00 UTC 2012

Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.

Blood

Gong G,Shao L,Wang Y,Chen CY,Huang D,Yao S,Zhan X,Sicard H,Wang R,Chen ZW

TCR2720 was used in flow cytometry to investigate the effect of phosphoantigen-activated V gamma 2V delta 2 T cells for interleukin-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection

Thu Jan 22 00:00:00 UTC 2009

Reciprocal interactions between human mesenchymal stem cells and gammadelta T cells or invariant natural killer T cells.

Stem cells (Dayton, Ohio)

Prigione I,Benvenuto F,Bocca P,Battistini L,Uccelli A,Pistoia V

TCR2720 was used in ELISA to investigate the interactions between mesenchymal stem cells and gammadelta T cells

Sun Mar 01 00:00:00 UTC 2009

Human CD1-restricted T cell recognition of lipids from pollens.

The Journal of experimental medicine

Agea E,Russano A,Bistoni O,Mannucci R,Nicoletti I,Corazzi L,Postle AD,De Libero G,Porcelli SA,Spinozzi F

TCR2720 was used in flow cytometry to study the immune response of pollen lipids

Mon Jul 18 00:00:00 UTC 2005

Recognition of nonpeptide antigens by human V gamma 9V delta 2 T cells requires contact with cells of human origin.

Clinical and experimental immunology

Green AE,Lissina A,Hutchinson SL,Hewitt RE,Temple B,James D,Boulter JM,Price DA,Sewell AK

TCR2720 was used in flow cytometry to study gamma delta T cell activation

Tue Jun 01 00:00:00 UTC 2004

Cytotoxic hepatosplenic gammadelta T-cell lymphoma following acute myeloid leukemia bearing two distinct gamma chains of the T-cell receptor. Biologic and clinical features.

Haematologica

Weidmann E,Hinz T,Klein S,Schui DK,Harder S,Kriener S,Kabelitz D,Hoelzer D,Mitrou PS

TCR2720 was used in flow cytometry to study the expression of Vd1 and two distinct TCRg chains, Vg3 and Vg9 during hepatosplenic gd-T-cell lymphoma

Sun Oct 01 00:00:00 UTC 2000

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TCR V gamma 9 Monoclonal Antibody (7A5), FITC

TCR V gamma 9 Antibody (TCR2720) in Flow

Product Details

TCR2720

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Performance Guarantee

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