Invitrogen

TCR gamma/delta Monoclonal Antibody (B1.1), eBioscience™

TCR gamma/delta Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

TCR gamma/delta Antibody (14-9959-82) in Flow

Surface staining of normal human peripheral blood cells with Anti-Human gamma delta TCR FITC (left) or PE (right).Appropriate isotype controls were used (open histogram).Cells in the lymphocyte gate were used for analysis.

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Product Details

14-9959-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 10 publications 10 publications

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

B1.1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_468641

Product Specific Information

Description: The B1.1 monoclonal antibody reacts with the human gamma delta TCR complex. The gamma delta TCR is expressed by a small subset of T cells in the thymus, peripheral lymphoid tissues, intestinal epithelium, and epidermis. The exact specificity, ligand and function of gamma delta TCR-bearing T cells are not yet fully understood; it is suggested that these cells recognize bacterial ligands and some tumor cells in the context of MHC class I-like gene products and play a role in regulation of the immune response and during bacterial infection. This monoclonal can be used as a phenotypic marker for gamma delta TCR-expressing T cells.

Applications Reported: The B1.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The B1.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). However, a small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.

Immunity

Szabo PA,Dogra P,Gray JI,Wells SB,Connors TJ,Weisberg SP,Krupska I,Matsumoto R,Poon MML,Idzikowski E,Morris SE,Pasin C,Yates AJ,Ku A,Chait M,Davis-Porada J,Guo XV,Zhou J,Steinle M,Mackay S,Saqi A,Baldwin MR,Sims PA,Farber DL

Published figure using TCR gamma/delta monoclonal antibody (Product # 14-9959-82) in Flow Cytometry

Tue Apr 13 00:00:00 UTC 2021

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Published figure using TCR gamma/delta monoclonal antibody (Product # 14-9959-82) in Flow Cytometry

Fri Jun 25 00:00:00 UTC 2021

Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in Adults.

Frontiers in immunology

Ben-Othman R,Cai B,Liu AC,Varankovich N,He D,Blimkie TM,Lee AH,Gill EE,Novotny M,Aevermann B,Drissler S,Shannon CP,McCann S,Marty K,Bjornson G,Edgar RD,Lin DTS,Gladish N,Maclsaac J,Amenyogbe N,Chan Q,Llibre A,Collin J,Landais E,Le K,Reiss SM,Koff WC,Havenar-Daughton C,Heran M,Sangha B,Walt D,Krajden M,Crotty S,Sok D,Briney B,Burton DR,Duffy D,Foster LJ,Mohn WW,Kobor MS,Tebbutt SJ,Brinkman RR,Scheuermann RH,Hancock REW,Kollmann TR,Sadarangani M

Published figure using TCR gamma/delta monoclonal antibody (Product # 14-9959-82) in Flow Cytometry

Mon Jun 21 00:00:00 UTC 2021

A non-canonical function of Ezh2 preserves immune homeostasis.

EMBO reports

Vasanthakumar A,Xu D,Lun AT,Kueh AJ,van Gisbergen KP,Iannarella N,Li X,Yu L,Wang D,Williams BR,Lee SC,Majewski IJ,Godfrey DI,Smyth GK,Alexander WS,Herold MJ,Kallies A,Nutt SL,Allan RS

14-9959 was used in Flow cytometry/Cell sorting to identify a chromatin-independent function of enhancer of zeste 2 that impacts on the development of the immune system.

Sat Apr 01 00:00:00 UTC 2017

Apoptosis Induced via Gamma Delta T Cell Antigen Receptor "Blocking" Antibodies: A Cautionary Tale.

Frontiers in immunology

Dutta I,Postovit LM,Siegers GM

14-9959 was used in Inhibition experiments to investigate the mechanisms beyond cytotoxicity inhibition following monoclonal antibody blocking of the γΔ T cell antigen receptor.

Wed Nov 20 00:00:00 UTC 2019

Quantitation of Productively Infected Monocytes and Macrophages of Simian Immunodeficiency Virus-Infected Macaques.

Journal of virology

Avalos CR,Price SL,Forsyth ER,Pin JN,Shirk EN,Bullock BT,Queen SE,Li M,Gellerup D,O'Connor SL,Zink MC,Mankowski JL,Gama L,Clements JE

14-9959 was used in Flow cytometry/Cell sorting to develop an assay to quantitate myeloid cells containing viral genomes that lead to productive infection in SIV-infected macaques.

Wed Jun 15 00:00:00 UTC 2016

Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.

Arthritis & rheumatology (Hoboken, N.J.)

Van Eyck L,De Somer L,Pombal D,Bornschein S,Frans G,Humblet-Baron S,Moens L,de Zegher F,Bossuyt X,Wouters C,Liston A

14-9959 was used in Flow cytometry/Cell sorting to determine the underlying genetic defect responsible for severe early onset and refractory systemic lupus erythematosus.

Mon Jun 01 00:00:00 UTC 2015

Thymic HIV-2 infection uncovers posttranscriptional control of viral replication in human thymocytes.

Journal of virology

Nunes-Cabaço H,Matoso P,Foxall RB,Tendeiro R,Pires AR,Carvalho T,Pinheiro AI,Soares RS,Sousa AE

14-9959 was used in Flow cytometry/Cell sorting to assess the direct impact of HIV-2 infection on the human thymus.

Sun Feb 01 00:00:00 UTC 2015

Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations.

Nature medicine

Schubert D,Bode C,Kenefeck R,Hou TZ,Wing JB,Kennedy A,Bulashevska A,Petersen BS,Schäffer AA,Grüning BA,Unger S,Frede N,Baumann U,Witte T,Schmidt RE,Dueckers G,Niehues T,Seneviratne S,Kanariou M,Speckmann C,Ehl S,Rensing-Ehl A,Warnatz K,Rakhmanov M,Thimme R,Hasselblatt P,Emmerich F,Cathomen T,Backofen R,Fisch P,Seidl M,May A,Schmitt-Graeff A,Ikemizu S,Salzer U,Franke A,Sakaguchi S,Walker LSK,Sansom DM,Grimbacher B

14-9959 was used in Flow cytometry/Cell sorting to demonstrate the consequences for mice with mutations in CTLA4, an essential negative regulator of immune responses.

Mon Dec 01 00:00:00 UTC 2014

Lipoxin A4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma.

Science translational medicine

Barnig C,Cernadas M,Dutile S,Liu X,Perrella MA,Kazani S,Wechsler ME,Israel E,Levy BD

14-9959 was used in Flow cytometry/Cell sorting to identify the potential of innate lymphoid cells to decrease airway inflammation.

Wed Feb 27 00:00:00 UTC 2013

Specific recruitment of γδ regulatory T cells in human breast cancer.

Cancer research

Ye J,Ma C,Wang F,Hsueh EC,Toth K,Huang Y,Mo W,Liu S,Han B,Varvares MA,Hoft DF,Peng G

14-9959 was used in Flow cytometry/Cell sorting to show how γδ Tregs accumulate in breast tumors, providing a rationale for their immunologic targeting to relieve immunosuppression in the tumor microenvironment.

Tue Oct 15 00:00:00 UTC 2013

Tumor-infiltrating γδ T lymphocytes predict clinical outcome in human breast cancer.

Journal of immunology (Baltimore, Md. : 1950)

Ma C,Zhang Q,Ye J,Wang F,Zhang Y,Wevers E,Schwartz T,Hunborg P,Varvares MA,Hoft DF,Hsueh EC,Peng G

14-9959 was used in Immunohistochemistry-immunofluorescence to suggest that intratumoural γδ T cells may serve as independent prognostic biomarkers, and potential therapeutic targets for human breast cancer.

Thu Nov 15 00:00:00 UTC 2012

IL-15 transpresentation promotes both human T-cell reconstitution and T-cell-dependent antibody responses in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Huntington ND,Alves NL,Legrand N,Lim A,Strick-Marchand H,Mention JJ,Plet A,Weijer K,Jacques Y,Becker PD,Guzman C,Soussan P,Kremsdorf D,Spits H,Di Santo JP

14-9959 was used in Flow cytometry/Cell sorting to investigate the role of transpresented IL-15 on human T-cell development and homeostasis.

Tue Apr 12 00:00:00 UTC 2011

The NKG2D ligand ULBP4 binds to TCRgamma9/delta2 and induces cytotoxicity to tumor cells through both TCRgammadelta and NKG2D.

Blood

Kong Y,Cao W,Xi X,Ma C,Cui L,He W

14-9959 was used in Blocking experiments to investigate whether UL16-binding protein 4 could be recognised by gammadeltaT cells via TCRgammadelta.

Thu Jul 09 00:00:00 UTC 2009

Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys.

Infection and immunity

Deetz CO,Hebbeler AM,Propp NA,Cairo C,Tikhonov I,Pauza CD

Tue Aug 01 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: T3/TCR complex; TCR delta gamma; TCR gamma/delta; TCR-CD3 complex

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Gene Aliases: TCRD; TCRDV1; TRD@

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Entrez Gene ID: (Human) 6964

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TCR gamma/delta Monoclonal Antibody (B1.1), eBioscience™

TCR gamma/delta Antibody (14-9959-82) in Flow

Product Details

14-9959-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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