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TDG Antibody (MA5-31519) in WB
TDG Polyclonal Antibody detects TDG protein by western blot analysis.293T mock transfected (-) or transfected with a construct expressing human TDG.10 % SDS-PAGE. TDG Polyclonal Antibody TDG Monoclonal Antibody (GT622) (Product # MA5-31519) dilution: 1:10,000.
Product Details
MA5-31519
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GT622
Immunogen
Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2787150
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Human (99%), Mouse (88%), Rat (87%), Bovine (91%).
Positive Control: human TDG-transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mis paired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G/T mismatch-specific thymine DNA glycosylase; hTDG; Thymine-DNA glycosylase
Gene Aliases: hTDG; TDG
UniProt ID: (Human) Q13569
Entrez Gene ID: (Human) 6996
RNA polymerase II transcription cofactor activity
damaged DNA binding
double-stranded DNA binding
uracil DNA N-glycosylase activity
protein kinase C binding
protein binding
pyrimidine-specific mismatch base pair DNA N-glycosylase activity
DNA N-glycosylase activity
mismatched DNA binding
SUMO binding
protein homodimerization activity
structure-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
base-excision repair
base-excision repair, AP site formation
mismatch repair
transcription, DNA-templated
embryo development
covalent chromatin modification
protein sumoylation
negative regulation of protein binding
oxidative DNA demethylation
negative regulation of chromatin binding
regulation of gene expression, epigenetic
depyrimidination
DNA demethylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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