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Invitrogen
TEAD1 Polyclonal Antibody
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FIGURE: 1 / 6
TEAD1 Antibody (PA5-106477) in ICC/IF
Immunocytochemistry analysis of TEAD1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with TEAD1 polyclonal antibody (Product # PA5-106477) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106477
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TEAD1(Accession P28347), corresponding to amino acid residues R75-M125.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854146
Product Specific Information
Antibody detects endogenous levels of total TEAD1.
Target Information
TEF1 (Transcriptional enhancer factor 1), a member of the TEA/ATTS domain family, is a nuclear protein that is expressed in numerous cell types and plays a role in controlling the expression of numerous genes. TEF family members have a highly conserved DNA-binding domain TEF-1 binds to GT-IIC, SphI/II and M-CAT. TEF-1 also binds to the proximal regulatory element (PRE) of transforming growth factor-alpha, a member of the EGF family that is overexpressed in many types of cancer. Furthermore, TEF-1 represses transcription in placental cells. In vitro, TEF-1 is phosphorylated by several PKC isozymes. TEF-1 is phosphorylated in vivo at serine and threonine residues. Phosphorylation of TEF-1, both in vivo and in vitro, results in a reduction in its DNA-binding capability, which suggests a potential role for TEF-1 in PKC inhibition. TEF-1 also complexes with larger tumor antigen (TAg), and may thus have a role in tumorigenesis. Dimerization of TEF-1 may be important for TEF-1 to function as a regulator of gene transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: N TEAD1; NTEF-1; NTEF1; Protein GT-IIC; TCF-13; TEA domain family member 1; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEAD-1; Transcription factor 13; transcriptional enhancer factor 1; Transcriptional enhancer factor TEF-1
Gene Aliases: 2610024B07Rik; AA; B230114H05Rik; Gtrgeo5; mTEF-1; NTEF-1; REF1; TCF-13; TCF13; TEAD-1; TEAD1; TEF-1; TEF1
UniProt ID: (Human) P28347
Entrez Gene ID: (Human) 7003, (Mouse) 21676, (Rat) 361630
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
core promoter proximal region DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
embryonic heart tube morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
heart development
transition between fast and slow fiber
notochord development
hippo signaling
regulation of cell proliferation
positive regulation of transcription from RNA polymerase II promoter
paraxial mesoderm development
lateral mesoderm development
cellular response to retinoic acid
positive regulation of protein serine/threonine kinase activity
positive regulation of stem cell population maintenance
transcription initiation from RNA polymerase II promoter
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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