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TEF Antibody (PA5-106495) in ICC/IF
Immunofluorescent analysis of TEF in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TEF polyclonal antibody (Product # PA5-106495) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-106495
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TEF(Accession Q10587), corresponding to amino acid residues R204-R254.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854164
Product Specific Information
Antibody detects endogenous levels of total TEF.
Target Information
TEF (thyrotrophic embryonic factor), also known as KIAA1655, is a 303 amino acid nuclear transcription factor that belongs to the bZIP (basic region/leucine zipper) family and PAR (proline and acidic amino acid-rich) subfamily. TEF binds DNA as either a homodimer or heterodimer, and is known to transactivate the TSH beta promoter. While broadly expressed in adults, TEF is only found in developing embryonic anterior pituitary gland. TEF accumulates according to a robust circadian rhythm and has also been found to inhibit cell growth by downregulating beta chain expression of cytokine receptors. The functional domains of TEF are highly homologous with other members of the PAR-bZIP subfamily, including albumin D box-binding protein (DABP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Thyrotroph embryonic factor; thyrotrophic embryonic factor
Gene Aliases: 2310028D20Rik; KIAA1655; TEF
UniProt ID: (Human) Q10587, (Rat) P41224, (Mouse) Q9JLC6
Entrez Gene ID: (Human) 7008, (Rat) 29362, (Mouse) 21685
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
double-stranded DNA binding
protein homodimerization activity
sequence-specific DNA binding
protein heterodimerization activity
DNA binding
transcription factor activity, sequence-specific DNA binding
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