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FIGURE: 1 / 1
TEF Antibody (PA5-78195) in WB
Western Blot using TEF Polyclonal Antibody (Product # PA5-78195). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TEF Polyclonal Antibody (Product # PA5-78195) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-78195
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human TEF. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.91 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2736695
Product Specific Information
Positive Control: TEF-transfected 293T
Predicted Reactivity: Mouse (97%), Rat (98%), Sheep (95%), Rhesus Monkey (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TEF (thyrotrophic embryonic factor), also known as KIAA1655, is a 303 amino acid nuclear transcription factor that belongs to the bZIP (basic region/leucine zipper) family and PAR (proline and acidic amino acid-rich) subfamily. TEF binds DNA as either a homodimer or heterodimer, and is known to transactivate the TSH beta promoter. While broadly expressed in adults, TEF is only found in developing embryonic anterior pituitary gland. TEF accumulates according to a robust circadian rhythm and has also been found to inhibit cell growth by downregulating beta chain expression of cytokine receptors. The functional domains of TEF are highly homologous with other members of the PAR-bZIP subfamily, including albumin D box-binding protein (DABP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Thyrotroph embryonic factor; thyrotrophic embryonic factor
Gene Aliases: KIAA1655; TEF
UniProt ID: (Human) Q10587
Entrez Gene ID: (Human) 7008
Performance Guarantee
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