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Invitrogen
TEF1 Monoclonal Antibody (GT13112)
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FIGURE: 1 / 5
TEF1 Antibody (MA5-27786) in ICC/IF
TEF1 Monoclonal Antibody (GT13112) detects TEF1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEF1 protein stained by TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786) diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.
Product Details
MA5-27786
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GT13112
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TEF1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735391
Product Specific Information
Positive Control: HeLa NE
Predicted Reactivity: Pig (100%), Chicken (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TEF1 (Transcriptional enhancer factor 1), a member of the TEA/ATTS domain family, is a nuclear protein that is expressed in numerous cell types and plays a role in controlling the expression of numerous genes. TEF family members have a highly conserved DNA-binding domain TEF-1 binds to GT-IIC, SphI/II and M-CAT. TEF-1 also binds to the proximal regulatory element (PRE) of transforming growth factor-alpha, a member of the EGF family that is overexpressed in many types of cancer. Furthermore, TEF-1 represses transcription in placental cells. In vitro, TEF-1 is phosphorylated by several PKC isozymes. TEF-1 is phosphorylated in vivo at serine and threonine residues. Phosphorylation of TEF-1, both in vivo and in vitro, results in a reduction in its DNA-binding capability, which suggests a potential role for TEF-1 in PKC inhibition. TEF-1 also complexes with larger tumor antigen (TAg), and may thus have a role in tumorigenesis. Dimerization of TEF-1 may be important for TEF-1 to function as a regulator of gene transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: N TEAD1; NTEF-1; NTEF1; Protein GT-IIC; TCF-13; TEA domain family member 1; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEAD-1; Transcription factor 13; transcriptional enhancer factor 1; Transcriptional enhancer factor TEF-1
Gene Aliases: AA; NTEF-1; REF1; TCF-13; TCF13; TEAD-1; TEAD1; TEF-1; TEF1
UniProt ID: (Human) P28347
Entrez Gene ID: (Human) 7003
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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