Invitrogen

TERT Monoclonal Antibody (2C4)

1 Published Figure

5 References

View all (37) TERT antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (1)

TERT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

TERT Antibody (MA5-16034) in IHC (P)

Immunohistochemical analysis of TERT in Normal human pancreas. Samples were incubated in TERT monoclonal antibody (Product # MA5-16034). Showing moderate staining of exocrine cells and a subset of islets of Langerhans.

TERT Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

MA5-16034

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

- -

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1:50-1:200

Immunoprecipitation (IP)

1:10-1:500

ChIP assay (ChIP)

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Cynomolgus monkey, Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

2C4

Immunogen

Full-length recombinant hTERT from insect cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

IgM

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11153210

Product Specific Information

Suggested positive control: 293T whole cell lysates, HeLa whole cell lysates, antigen standard for TERT (transient overexpression lysate).

Target Information

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase (TERT) have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nuclear and Cytoplasmic hTERT, Tumor-Infiltrating Lymphocytes, and Telomere Elongation Leukocytes Are Independent Factors in the Response to Neoadjuvant Treatment in HER2-Enriched Breast Cancer.

Current oncology (Toronto, Ont.)

Delmonico L,Bines J,Nascimento CMD,Fernandes PV,Barbosa IS,Ribeiro GB,de Paula BHR,Silvestre RT,Ornellas MHF,Alves G,Lage C

MA5-16034 was used in Immunohistochemistry to evaluate the presence of tumor-infiltrating lymphocytes, hTERT expression, hTERT promoter mutation, and leukocyte telomere length in HER2-enriched breast tumors.

Fri Apr 07 00:00:00 UTC 2023

The Synthetic Flavonoid Hidrosmin Improves Endothelial Dysfunction and Atherosclerotic Lesions in Diabetic Mice.

Antioxidants (Basel, Switzerland)

Jiménez-Castilla L,Opazo-Ríos L,Marin-Royo G,Orejudo M,Rodrigues-Diez R,Ballesteros-Martínez C,Soto-Catalán M,Caro-Ordieres T,Artaiz I,Suarez-Cortés T,Zazpe A,Hernández G,Cortés M,Tuñón J,Briones AM,Egido J,Gómez-Guerrero C

MA5-16034 was used in Immunohistochemistry to highlight hidrosmin as a potential add-on therapy in the treatment of macrovascular complications of diabetes.

Mon Dec 19 00:00:00 UTC 2022

Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy.

Translational vision science & technology

Liu YC,Hall B,Lwin NC,Teo EPW,Yam GHF,Hipsley A,Mehta JS

MA5-16034 was used in Immunohistochemistry to demonstrate the course of inflammatory and wound-healing responses following laser scleral microporation.

Sun Mar 01 00:00:00 UTC 2020

Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy.

Translational vision science & technology

Liu YC,Hall B,Lwin NC,Teo EPW,Yam GHF,Hipsley A,Mehta JS

MA5-16034 was used in Immunohistochemistry to demonstrate the course of inflammatory and wound-healing responses following laser scleral microporation.

Sun Mar 01 00:00:00 UTC 2020

Telomerase Reverse Transcriptase Deficiency Prevents Neointima Formation Through Chromatin Silencing of E2F1 Target Genes.

Arteriosclerosis, thrombosis, and vascular biology

Endorf EB,Qing H,Aono J,Terami N,Doyon G,Hyzny E,Jones KL,Findeisen HM,Bruemmer D

MA516034 was used in immunohistochemistry - paraffin section to test if telomerase reverse transcriptase modulates proliferative vascular remodeling

Wed Feb 01 00:00:00 UTC 2017

The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma.

Journal of oral science

Carkic J,Nikolic N,Radojevic-Skodric S,Kuzmanovic-Pficer J,Brajovic G,Antunovic M,Milasin J,Popovic B

MA516034 was used in immunohistochemistry - paraffin section to determine the relationship of a hTERT polymorphism in the development of oral squamous cell carcinoma.

Mon Nov 13 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: HEST2; hTERT; Telomerase catalytic subunit; Telomerase reverse transcriptase; telomerase reverse transcriptase beta; Telomerase-associated protein 2; tert {ECO:0000312|RGD:70494}; TP2

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Gene Aliases: CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TERT; TP2; TR; TRT

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UniProt ID: (Human) O14746, (Rat) Q673L6, (Mouse) O70372

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Entrez Gene ID: (Human) 7015, (Rat) 301965, (Mouse) 21752

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Function(s)

tRNA binding transcription coactivator binding DNA binding telomerase activity telomerase RNA reverse transcriptase activity RNA binding RNA-directed DNA polymerase activity RNA-directed RNA polymerase activity protein binding nucleotidyltransferase activity telomeric DNA binding protein homodimerization activity metal ion binding telomerase RNA binding transferase activity

Process(es)

transcription, RNA-templated obsolete age-dependent telomere shortening RNA-dependent DNA biosynthetic process telomere maintenance via telomerase mitochondrion organization biological_process negative regulation of gene expression DNA strand elongation positive regulation of Wnt signaling pathway production of siRNA involved in RNA interference telomere formation via telomerase positive regulation of hair cycle positive regulation of nitric-oxide synthase activity cellular response to hypoxia DNA biosynthetic process replicative senescence positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of production of siRNA involved in RNA interference positive regulation of stem cell proliferation negative regulation of cellular senescence negative regulation of extrinsic apoptotic signaling pathway in absence of ligand telomere maintenance regulation of protein stability positive regulation of protein binding RNA biosynthetic process establishment of protein localization to telomere positive regulation of protein localization to nucleolus beta-catenin-TCF complex assembly

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TERT Monoclonal Antibody (2C4)

TERT Antibody (MA5-16034) in IHC (P)

Product Details

MA5-16034

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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