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Invitrogen
TEV Cleavage Site Monoclonal Antibody (9A10-4C3)
FIGURE: 1 / 1
TEV Cleavage Site Antibody (MA1-124) in WB
Western blot analysis of TEV cleavage site was performed by loading the indicated amounts of TEV control protein and 5 µL of Lane Marker Reducing Sample Buffer (Product # 39000) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a TEV cleavage site monoclonal antibody (Product # MA1-124) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HR... View More
Product Details
MA1-124
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
9A10-4C3
Immunogen
KLH conjugate peptide representing the cleavage site recognized by TEV enzyme
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536832
Product Specific Information
MA1-124 has been successfully used in Western blot applications.
The MA1-124 immunogen is a peptide representing the cleavage site recognized by TEV enzyme.
Target Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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