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TEV Protease Antibody (200-401-B91) in WB
E.coli lysate containing recombinant TEV protease was loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 1% BSA (p/n BSA-30) in TBS for 30min at ambient. The membrane was probed with the primary antibody at a 1:1,000 dilution in 1%BSA/TBS overnight at 4° C. For detection HRP conjugated Gt-a-Rabbit IgG (p/n 611-103-122) was used at a 1:40,000 dilution for 30 min at ambient and data generated with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using Bio-Rad ... View More
Product Details
200-401-B91
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant MBP-and-poly-His-tagged auto inactivation-resistant mutant TEV Protease.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Assay by western blot showed no reactivity to MBP.
Target Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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