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Invitrogen
TFC4 Polyclonal Antibody
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TFC4 Antibody (PA5-144490) in WB
Western blot analysis of TFC4 in recombinant protein using a dilution of 1:2,000. Samples were incubated with TFC4 polyclonal antibody (Product # PA5-144490). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 54.9 kDa. Observed band size: 61 kDa.
Product Details
PA5-144490
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508/S288c) (Baker's yeast) TFC4 protein
Conjugate
Form
Liquid
Concentration
2.755 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_3091715
Target Information
TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes. TFC4 is the TFIIIB-assembling subunit of TFIIIC and essential for viability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PCF1; Tfc4p; TFIIIC 131 kDa subunit; Transcription factor C subunit 4; Transcription factor tau 131 kDa subunit
Gene Aliases: PCF1; TFC4; YGR047C
UniProt ID: (Baker's yeast) P33339
Entrez Gene ID: (Baker's yeast) 852938
RNA polymerase III type 1 promoter sequence-specific DNA binding
RNA polymerase III type 2 promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase III type 1 promoter sequence-specific binding, TFIIIB recruiting
transcription factor activity, RNA polymerase III type 2 promoter sequence-specific binding, TFIIIB recruiting
DNA binding
DNA binding, bending
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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